Preparation method of immobilized slow-release microorganisms for in-situ remediation of water body
An in-situ remediation and microbial technology, applied in chemical instruments and methods, water pollutants, biological water/sewage treatment, etc. problems, to achieve the effect of being conducive to denitrification of river water and degradation of organic matter, maintaining microbial activity, and improving microbial activity
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[0031] Microbial adsorption: Wash the molecular sieve particles with ultrapure water, soak the molecular sieve particles in the prepared denitrification functional bacteria solution for 3 cycles, 5 days per cycle, 15 days in total, and maintain the temperature at 25°C.
[0032] Chitosan solution configuration: prepare a volume ratio of 1% glacial acetic acid solution, weigh a certain amount of chitosan (deacetylation degree ≥ 90%) and add it to 1% glacial acetic acid solution to make the chitosan mass concentration 1.5% , 35 ° C water bath for 20 minutes, stirring and dissolving to make a chitosan solution.
[0033] Chitosan coating: Take out the molecular sieve carrier that has adsorbed microorganisms from the bacterial solution, immerse in the chitosan solution and cover the film. After 20 minutes of coating, take out the carrier and add 0.25mol / L Na 2 SO 4 solution, fixed for 20 min, removed, washed with ultrapure water three times and refrigerated for later use.
[0034]...
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