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Preparation method of immobilized slow-release microorganisms for in-situ remediation of water body

An in-situ remediation and microbial technology, applied in chemical instruments and methods, water pollutants, biological water/sewage treatment, etc. problems, to achieve the effect of being conducive to denitrification of river water and degradation of organic matter, maintaining microbial activity, and improving microbial activity

Inactive Publication Date: 2020-04-21
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limitation of the material itself in the embedding method, it is difficult to maintain the stability of the immobilized balls in the face of complex and changeable river water, and it is easy to swell or even break after long-term immersion in the river water, and even bring secondary pollution to the water body

Method used

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  • Preparation method of immobilized slow-release microorganisms for in-situ remediation of water body
  • Preparation method of immobilized slow-release microorganisms for in-situ remediation of water body
  • Preparation method of immobilized slow-release microorganisms for in-situ remediation of water body

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Effect test

Embodiment 1

[0031] Microbial adsorption: Wash the molecular sieve particles with ultrapure water, soak the molecular sieve particles in the prepared denitrification functional bacteria solution for 3 cycles, 5 days per cycle, 15 days in total, and maintain the temperature at 25°C.

[0032] Chitosan solution configuration: prepare a volume ratio of 1% glacial acetic acid solution, weigh a certain amount of chitosan (deacetylation degree ≥ 90%) and add it to 1% glacial acetic acid solution to make the chitosan mass concentration 1.5% , 35 ° C water bath for 20 minutes, stirring and dissolving to make a chitosan solution.

[0033] Chitosan coating: Take out the molecular sieve carrier that has adsorbed microorganisms from the bacterial solution, immerse in the chitosan solution and cover the film. After 20 minutes of coating, take out the carrier and add 0.25mol / L Na 2 SO 4 solution, fixed for 20 min, removed, washed with ultrapure water three times and refrigerated for later use.

[0034]...

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Abstract

The invention belongs to the technical field of water treatment, and particularly relates to a preparation method of immobilized slow-release microorganisms for in-situ remediation of a water body. The preparation method comprises the following steps: soaking molecular sieve particles in a denitrification functional bacteria solution to absorb a large amount of denitrification functional bacteriato prepare immobilized microorganism inner cores, and coating the surfaces of the inner cores with chitosan to prepare the chitosan coated immobilized slow-release microorganisms. Compared with the prior art, the immobilized slow-release microorganism particles prepared by the preparation method have relatively high stability, can stably purify a water body for a long time, prolong the service lifetime of the immobilized slow-release microorganisms and avoid secondary pollution of the water body.

Description

technical field [0001] The invention belongs to the technical field of water treatment, and in particular relates to a preparation method of immobilized slow-release microorganisms that can be used for in-situ restoration of water bodies. Background technique [0002] More than 50,000 river basins in my country have an area greater than 100km 2 Most of the rivers are polluted to varying degrees. Although the use of microorganisms with specific functions can effectively repair water bodies and improve water quality, there are problems such as easy loss of bacterial cells, large dosage, and susceptibility to water conservancy conditions in free biological agents. [0003] Immobilized slow-release microbial technology can effectively solve the problems of free biological agents. The embedding method is a relatively common method of immobilizing microorganisms at present, which can effectively maintain the activity of microorganisms. Commonly used materials include polyvinyl a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F1/28C02F101/16C02F101/30
CPCC02F1/281C02F3/34C02F2101/16C02F2101/30C02F2103/007C02F2305/06
Inventor 李茹莹张泽钰刘玉佳
Owner TIANJIN UNIV
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