A kind of preparation method of intelligent high-efficiency antibacterial agent based on protein

An antibacterial agent and protein technology, applied in the field of the preparation of intelligent and efficient antibacterial agents, can solve the problems of difficult treatment of drug-resistant bacteria, low sterilization efficiency, poor biocompatibility, etc., and achieve the goal of improving biocompatibility and drug resistance. The effect of reducing the chance and prolonging the half-life

Active Publication Date: 2022-06-07
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims to solve the problems of low bactericidal efficiency, poor biocompatibility and difficult treatment of drug-resistant bacteria of traditional antibacterial agents, and provides a method for preparing an intelligent and efficient antibacterial agent based on protein

Method used

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  • A kind of preparation method of intelligent high-efficiency antibacterial agent based on protein
  • A kind of preparation method of intelligent high-efficiency antibacterial agent based on protein
  • A kind of preparation method of intelligent high-efficiency antibacterial agent based on protein

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specific Embodiment approach 1

[0025] Embodiment 1: The preparation method of a protein-based intelligent and efficient antibacterial agent of the present embodiment is specifically carried out according to the following steps:

[0026] 1. Construction of antibacterial agent skeleton: BSA solution was prepared under magnetic stirring; hexamethylenediamine was dissolved in water to prepare hexamethylenediamine solution, and the pH of hexamethylenediamine solution was adjusted to 4.5~ with dilute hydrochloric acid 7.5, add the hexamethylenediamine solution after adjusting the pH dropwise to the BSA solution at a rate of 0.5~3mL / min to obtain a reaction solution, and then add 1-ethyl-3-(3-dimethylamine to the reaction solution) propyl) carbodiimide hydrochloride solution, react at room temperature for 10h, after the reaction is completed, filter, dialyze, freeze-dry to obtain BSA-NH 2 ; The concentration of the BSA solution is 5~20 mg / mL; The concentration of the hexamethylenediamine solution is 0.1~0.5 mg / mL;...

specific Embodiment approach 2

[0033] Embodiment 2: This embodiment differs from Embodiment 1 in that: in step 1, dilute hydrochloric acid is used to adjust the pH of the hexamethylenediamine solution to 6.5; in step 2, the pH of the guanidinoacetic acid solution is adjusted to 6.5. Others are the same as the first embodiment.

specific Embodiment approach 3

[0034] Embodiment 3: This embodiment differs from Embodiment 1 or 2 in that: the concentration of the BSA solution in step 1 is 10 mg / mL; the concentration of the hexamethylenediamine solution is 0.2 mg / mL; the The concentration of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride solution was 50 mg / mL. Others are the same as in the first or second embodiment.

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Abstract

A method for preparing a protein-based intelligent and efficient antibacterial agent. The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method of a protein-based intelligent high-efficiency antibacterial agent. The purpose of the invention is to solve the problems of low bactericidal efficiency, poor biocompatibility and difficult treatment of drug-resistant bacteria of traditional antibacterial agents. Method: Using macromolecular protein as the skeleton, introducing small molecules with high-efficiency bactericidal properties, and at the same time improving the protein skeleton in order to obtain antibacterial agents with better biocompatibility, and at the same time play the role of directional intelligent sterilization. The macromolecular skeleton is not easy to produce drug resistance, and at the same time, it is easy to design broad-spectrum fungicides, which solves the problem that bacteria are prone to drug resistance. The introduction of it solves the problem of low sterilization efficiency of traditional antibacterial agents, solves the problem of difficulty in eliminating traditional antibacterial agents in organisms, and plays a special effect of intelligent sterilization.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method of a protein-based intelligent and efficient antibacterial agent. Background technique [0002] Since the golden age of the discovery of antibiotic small molecules (1840s to 1960s), various antibacterial small molecules have been used to fight infection or death caused by infectious diseases, and antibiotics have always been a powerful weapon for human beings to resist infectious diseases. These bactericidal small molecules kill bacteria by specifically binding to bacterial targets. However, with the large-scale use of small-molecule bactericides, the environmental pressure of antibiotics on bacteria has been formed, coupled with the short life cycle and genetic The characteristics of horizontal transfer, and the consequent emergence of drug-resistant microbial populations. In recent years, the phenomenon of bacterial resistance has become in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/64A61K47/60A61K31/198A61P31/04
CPCA61K47/643A61K47/60A61K31/198A61P31/04
Inventor 黄鑫方野刘小曼
Owner HARBIN INST OF TECH
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