Protein aqueous solution spraying equipment

Abstract

The invention relates to the field of processing and preparation of biomedical materials, and provides protein aqueous solution spraying equipment. The protein aqueous solution spraying equipment comprises a sealed tank, wherein a humidity adjusting device and a first temperature adjusting device are arranged on the sealed tank, a liquid storage tank and spraying equipment are arranged in the sealed tank, first filter screens and a second temperature adjusting device are arranged on the liquid storage tank, first driving equipment is arranged on the sealed tank, and the spraying equipment comprises a spray head and second driving equipment. The protein aqueous solution spraying equipment guarantees that the storage and spraying processes of a protein aqueous solution are in a constant-temperature and constant-humidity environment, a small amount of denatured protein is filtered out through the first filter screens, the phenomenon of blockage of the spray head is avoided, the separationof the protein aqueous solution from inorganic materials is avoided through shaking of the liquid storage tank, and the spraying effect is guaranteed. Meanwhile, the protein aqueous solution is uniformly sprayed on a sample through the spray head, and the smoothness of the surface of a coating is guaranteed, and the thickness of the coating can be changed according to the reciprocating times of the spray head, so that the coating meeting the requirements is obtained.

Description

technical field [0001] The invention relates to the field of processing and preparation of biomedical materials, in particular to a protein aqueous solution spraying equipment. Background technique [0002] Silk fibroin is extracted from silk or spider silk. It has a wide range of sources, convenient and diverse processing, and the product has excellent mechanical properties, biocompatibility, and biodegradability. Due to the characteristics that silk fibroin is easily denatured by temperature, humidity, stress, and concentration, the production method of the coating is relatively limited. The commonly used methods for making silk fibroin film layer in the prior art include sol-gel method, spin coating method, coating-evaporation method and so on. [0003] Due to the variability of silk fibroin, basically all silk fibroin coating methods tend to simply apply silk fibroin aqueous solution to the surface of the sample. However, these methods inevitably have many disadvantage...

AI Technical Summary

Problems solved by technology

However, these methods inevitably have many disadvantages: (1) firstly, due to the methods of coating, casting, soaking, etc., it is not easy to precisely control the thickness of the film layer, and the film layer obtained by this method is easy to crack and fall off; (2) If vertical dip coating is used, due to the influence of gravity and liquid surface tension, it is difficult to achieve uniform coverage of the coating; (3) Since the coating material is liquid and directly contacts the surface of the sample, it is difficult to achieve a more delicate coating At the same time, it relies more on the viscosity of the liquid to remain on the surface of the sample. In the coating process for unconventional flat samples, these shortcomings will be further amplified; (4) The coating solution itself is easily affected by temperature and humidity. Gel phenomenon is easy to occur during use or storage, which reduces the preparation efficiency; (5) in the forming stage of the coating, the water evaporation of the sprayed silk fibroin solution is slow, and it takes a long time to dry naturally, which is not suitable for mass production

[0004] Spraying is a commonly used coating preparation technology, which has been widely used in the industry. However, in the spraying of silk fibroin coating, because the silk fibroin solution is affected by the drastic changes in temperature, humidity and air flow in the spray gun, it is easy to cause silk fibroin. The protein structure changes, resulting in flocculent precipitates or gel substances, and the liquid stored in the spray gun tank is often denatured into gel due to the influence of humidity and temperature, which blocks the pipeline

At the same time, when inorganic materials are added to the silk fibroin solution to achieve specific functions, since the inorganic materials and the silk fibroin solution are fused in a physical state, the inorganic materials are separated from the silk fibroin solution under long-term precipitation. The resulting coating cannot achieve the desired effect

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