Protein water solution spraying equipment

Abstract

The invention relates to the field of biomedical material processing and preparation, and provides a protein aqueous solution spraying equipment. The spraying equipment includes a sealed tank, a humidity adjustment device and a first temperature adjustment device are arranged on the sealed tank, a liquid storage tank and a spraying equipment are arranged in the sealed tank, a first filter screen and a second temperature adjustment device are arranged on the liquid storage tank , the sealed tank is provided with a first driving device; the spraying device includes a spray head and a second driving device. This application guarantees that the storage and spraying process of the protein water solution is in a constant temperature and humidity environment, and a small amount of denatured protein is filtered through the first filter to avoid the phenomenon of nozzle clogging, and the shaking of the liquid storage tank avoids the protein water solution and Inorganic materials are separated to ensure the spraying effect. At the same time, the protein aqueous solution is evenly sprayed on the sample through the nozzle to ensure the smoothness of the coating surface, and the thickness of the coating can be changed according to the number of reciprocating movements of the nozzle to obtain a coating that meets the requirements. Floor.

Description

technical field [0001] The invention relates to the field of processing and preparation of biomedical materials, in particular to a protein aqueous solution spraying equipment. Background technique [0002] Silk fibroin is extracted from silk or spider silk. It has a wide range of sources, convenient and diverse processing, and the product has excellent mechanical properties, biocompatibility, and biodegradability. Due to the characteristics that silk fibroin is easily denatured by temperature, humidity, stress, and concentration, the production method of the coating is relatively limited. The commonly used methods for making silk fibroin film layer in the prior art include sol-gel method, spin coating method, coating-evaporation method and so on. [0003] Due to the variability of silk fibroin, basically all silk fibroin coating methods tend to simply apply silk fibroin aqueous solution to the surface of the sample. However, these methods inevitably have many disadvantage...

AI Technical Summary

Problems solved by technology

However, these methods inevitably have many disadvantages: (1) firstly, due to the methods of coating, casting, soaking, etc., it is not easy to precisely control the thickness of the film layer, and the film layer obtained by this method is easy to crack and fall off; (2) If vertical dip coating is used, due to the influence of gravity and liquid surface tension, it is difficult to achieve uniform coverage of the coating; (3) Since the coating material is liquid and directly contacts the surface of the sample, it is difficult to achieve a more delicate coating At the same time, it relies more on the viscosity of the liquid to remain on the surface of the sample. In the coating process for unconventional flat samples, these shortcomings will be further amplified; (4) The coating solution itself is easily affected by temperature and humidity. Gel phenomenon is easy to occur during use or storage, which reduces the preparation efficiency; (5) in the forming stage of the coating, the water evaporation of the sprayed silk fibroin solution is slow, and it takes a long time to dry naturally, which is not suitable for mass production

[0004] Spraying is a commonly used coating preparation technology, which has been widely used in the industry. However, in the spraying of silk fibroin coating, because the silk fibroin solution is affected by the drastic changes in temperature, humidity and air flow in the spray gun, it is easy to cause silk fibroin. The protein structure changes, resulting in flocculent precipitates or gel substances, and the liquid stored in the spray gun tank is often denatured into gel due to the influence of humidity and temperature, which blocks the pipeline

At the same time, when inorganic materials are added to the silk fibroin solution to achieve specific functions, since the inorganic materials and the silk fibroin solution are fused in a physical state, the inorganic materials are separated from the silk fibroin solution under long-term precipitation. The resulting coating cannot achieve the desired effect

Images