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Method for determining concentrations of prednisone, prednisone acetate and active metabolite prednisolone thereof in blood plasma by means of liquid chromatography-mass spectrometry

A technology for prednisone acetate and metabolites, which is applied in measurement devices, instruments, scientific instruments, etc., to achieve the effects of simple pretreatment method, simple operation and high sensitivity

Active Publication Date: 2020-04-24
SUZHOU GUOCHEN BIOTEK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no literature report on a method for simultaneous detection of prednisone, prednisone acetate and its active metabolite prednisolone

Method used

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  • Method for determining concentrations of prednisone, prednisone acetate and active metabolite prednisolone thereof in blood plasma by means of liquid chromatography-mass spectrometry
  • Method for determining concentrations of prednisone, prednisone acetate and active metabolite prednisolone thereof in blood plasma by means of liquid chromatography-mass spectrometry
  • Method for determining concentrations of prednisone, prednisone acetate and active metabolite prednisolone thereof in blood plasma by means of liquid chromatography-mass spectrometry

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Experimental program
Comparison scheme
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Embodiment

[0032] 1. Experimental materials and instruments

[0033] Prednisone, prednisone acetate and its metabolite prednisolone, as well as various deuterated internal standards prednisone-d8, prednisone acetate-d3, and prednisolone-d8 were all provided by Canadian TRC. Acetonitrile, methanol, acetic acid, etc. are HPLC grade, from Fisher Company of the United States, and other chemical reagents are analytically pure.

[0034] LCMS-8060 mass spectrometer (Shimadzu, Japan), Lab Solutions 6.84 SP1 data acquisition software (Shimadzu, Japan), Mettler XS 105DU electronic balance (Mettler, Switzerland); Eppendorf Centrifuge5424R high-speed low-temperature centrifuge ( Eppendorf, Germany).

[0035] 2. Experimental process

[0036] 1. Preparation of stock solution and working solution

[0037] Weigh an appropriate amount of prednisone, prednisone acetate and its metabolite prednisolone and various deuterated internal standards prednisone-d8, prednisone acetate-d3, prednisolone-d8, add a ...

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Abstract

The invention provides a method for determining concentrations of prednisone, prednisone acetate and active metabolite prednisolone thereof in blood plasma by means of liquid chromatography-mass spectrometry. The method comprises the steps of: (1) pretreating a blood plasma sample; (2) detecting the blood plasma sample by adopting liquid chromatography-mass spectrometry; (3) preparing a standard curve; and (4) determining the concentrations of prednisone, prednisone acetate and active metabolite prednisolone thereof in the blood plasma. The pretreatment method disclosed by the invention is simple, high in specificity and high in sensitivity, and the method is rapid, precise, high in sensitivity, simple in operation and suitable for clinical application of medicines.

Description

technical field [0001] The invention relates to the technical field of drug analysis, and relates to a method for analyzing and measuring drugs in vivo, in particular to a method for measuring the concentration of prednisone, prednisone acetate and its active metabolite prednisolone in blood plasma by liquid chromatography-mass spectrometry. Background technique [0002] Prednisone acetate belongs to the class of adrenal glucocorticoids. By inhibiting the accumulation of inflammatory cells, including macrophages and leukocytes at the site of inflammation, and inhibiting phagocytosis, the release of lysosomal enzymes and the synthesis and release of inflammatory chemical mediators, thereby reducing the manifestations of inflammation; by reducing T lymphocytes, The number of monocytes and eosinophils reduces the binding ability of immunoglobulins to cell surface receptors, and inhibits the synthesis and release of interleukins, thereby reducing the transformation of T lymphocy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/54
CPCG01N30/02G01N30/06G01N30/54G01N30/72G01N2030/027G01N2030/062
Inventor 王凯
Owner SUZHOU GUOCHEN BIOTEK CO LTD
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