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Beta-2-glycoprotein 1 derived peptide and use thereof for treating antiphospholipid syndrome

An anti-phospholipid and anti-phospholipid antibody technology is applied in the field of peptide separation, which can solve time-consuming and labor-intensive problems

Pending Publication Date: 2020-04-24
UNIVERSITY OF GENEVA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, although different tools are used, the diagnosis of APS patients is currently time-consuming and labor-intensive

Method used

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  • Beta-2-glycoprotein 1 derived peptide and use thereof for treating antiphospholipid syndrome
  • Beta-2-glycoprotein 1 derived peptide and use thereof for treating antiphospholipid syndrome
  • Beta-2-glycoprotein 1 derived peptide and use thereof for treating antiphospholipid syndrome

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Experimental program
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Effect test

Embodiment

[0134] Ethics statement

[0135] The Geneva Hospital Blood Transfusion Center provided buffy coats of healthy donors. In accordance with the Geneva Hospital Ethics Committee and the Declaration of Helsinki, the blood bank obtained informed consent from the donors, who were informed that a portion of their blood would be used for research purposes.

[0136] patient characteristics

[0137] All patients had APS as defined by the revised Sapporo criteria 2 . Control antibodies and peripheral blood mononuclear cells (PBMC) were isolated from the plasma of healthy volunteers. Table 1 lists the characteristics of the patients used in this study.

[0138] cell culture

[0139] Monocytes were isolated from buffy coats of healthy volunteers as previously described 14，15 . As assessed by flow cytometry, monocyte purity typically consists of >90% CD14 + cells, + cells and + cell composition. Cells were cultured in RPMI containing 10% fetal bovine serum (FBS; Gibco BRL-Life Techn...

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Abstract

The invention provides an isolated peptide containing a motif that binds antiphospholipid antibodies (aPLA) and that is recognized by CD4+ T cells that are able to induce production of antiphospholipid antibodies (aPLA). The present invention further provides methods for detection of aPLA and CD4+ T cells able to induce production of aPLA. The present invention also provides methods for treating the antiphospholipid syndrome (APS).

Description

technical field [0001] The present invention provides an isolated peptide comprising a motif that binds antiphospholipid antibody (aPLA) and is recognized by CD4+ T cells capable of inducing the production of antiphospholipid antibody (aPLA). The invention also provides a method for detecting aPLA and CD4+ T cells capable of inducing aPLA production. The present invention also provides methods of treating antiphospholipid syndrome (APS). Background technique [0002] Antiphospholipid syndrome (APS) has been described as a common risk factor for recurrent thromboembolic events and / or pregnancy complications due to circulating antiphospholipid antibodies (aPLA) 1 . The plasma phospholipid-binding protein β-2-glycoprotein 1 (β2GP1) is now generally accepted as the major antigenic target of aPLA 2 . β2GP1 is a 43 kDa protein consisting of five short consensus repeat domains called "sushi" domains. β2GP1 exists in two distinct conformations: a circular plasma conformation an...

Claims

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Application Information

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IPC IPC(8): G01N33/564A61K38/17
CPCA61K38/00C07K7/08G01N33/564G01N33/505G01N2800/24C07K7/06C07K14/70539
Inventor K·布兰特
Owner UNIVERSITY OF GENEVA