Construction method of hyperglycemic animal model

A technology of animal model and construction method, which is applied in the field of hyperglycemia animal model construction, can solve the problems of insulin insensitivity, long experiment period, and high mortality, and achieve the effects of increased survival rate, long experiment period, and reduced modeling cost

Inactive Publication Date: 2020-04-28
康美华大基因技术有限公司
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Problems solved by technology

The preparation of diabetic animal models is related to the way and dose of STZ injection. Large-dose injection can directly cause extensive destruction of pancreatic β-cells, resulting in a diabetic model, but there are problems of high mortality and poor molding rate; low-dose STZ

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  • Construction method of hyperglycemic animal model
  • Construction method of hyperglycemic animal model
  • Construction method of hyperglycemic animal model

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Experimental program
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Embodiment 1

[0041] Select 40 8-week-old SPF male C57BL / 6 mice, and use ear tags to distinguish the mice; at this time, the mice are sexually mature, the body is mature, and the tissues and organs are mature, which is convenient for the stability of the experimental data. Adopt blood glucose measuring instrument (Roche) to detect the fasting blood glucose of above-mentioned mouse, as basic blood glucose value;

[0042]The mice of this embodiment were injected intraperitoneally with 40 mg / kg·BW streptozotocin solution (4 mg streptozotocin / 1 ml citric acid-sodium citrate buffer) for 5 consecutive days, fasted for 4 h before each injection, and were free to Drinking water: on the third day after the injection, the mice were fasted for 6 hours, the tail was cut to collect venous blood, and the fasting blood sugar level was tested. At this time, the blood sugar level did not rise to the ideal value, and the mice were fasted for a total of 20 hours, and free to drink water during the period , an...

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Abstract

The present invention discloses a construction method of a hyperglycemic animal model. SPF grade C57BL/6 mice are selected, the mice are intraperitoneally injected with (38-42) mg/kg BW streptozotocinsolution for 5 consecutive days, and fasting for 3-5 h is conducted before injection; fasting for 18-22 h is conducted after 3 days and then intraperitoneal injection of (95-105) mg/kg BW streptozotocin solution is conducted once; and on the 3rd day after all injections are completed, fasting blood glucose value is measured and the mice with the blood glucose value greater than 12 mmol/L are typeI hyperglycemic animals. The suitable streptozotocin solution injection method is designed, administrations in multiple times are conducted, different sizes of injection dose, time interval and fasting time are used, high-fat and high-sugar feed and STZ combined induction is not needed, animals are high in survival rate, modeling period is short, modeling success rate is high, and the animal model has a stable blood glucose value and relatively low cost, and can be used as a research model for hypoglycemic drugs and pathogenesis of diabetes.

Description

technical field [0001] The invention relates to the field of biological model construction, in particular to a method for constructing a hyperglycemia animal model. Background technique [0002] The high incidence of diabetes and many complications have brought a heavy burden to human health and social development. The current treatment methods mainly use drugs or insulin injections to increase the secretion of insulin to lower blood sugar. The side effects of drugs are relatively high and the cost is high. Therefore, research on the treatment of diabetes has become a key research content for scientists. Among them, the animal model is an indispensable and important means and method for the study of diabetes, which will help to further reveal the pathogenesis and intervention measures of diabetes, and test and screen various hypoglycemic drugs. [0003] Studying the treatment of diabetes requires better animal models. At present, most diabetes models are genetically enginee...

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K2227/105A01K2267/0362
Inventor 王苗苗蒙洁妙张晓春陈星羽
Owner 康美华大基因技术有限公司
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