Organic colored microparticles, diagnostic reagent kit, and in vitro diagnosis method

A fine particle, organic technology, applied in the field of organic colored fine particles, can solve the problems of difficult to find TL, poor contrast, misdiagnosis, etc., and achieve the effect of full detection sensitivity and good reproducibility

Pending Publication Date: 2020-05-05
ASAHI KASEI KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the background is poor, the contrast between the nitrocellulose membrane and TL will be poor, and it will be difficult to find the colored lines of TL, making it difficult to judge in the medical field
Furthermore, if the background is poor, it is difficult to judge negative TL (ie, no color development)
These phenomena may lead to misdiagnosis

Method used

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  • Organic colored microparticles, diagnostic reagent kit, and in vitro diagnosis method
  • Organic colored microparticles, diagnostic reagent kit, and in vitro diagnosis method
  • Organic colored microparticles, diagnostic reagent kit, and in vitro diagnosis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0164] A cuprocellulose solution having a cellulose concentration of 0.37 wt%, a copper concentration of 0.13 wt%, and an ammonia concentration of 1.00 wt% was produced by a conventionally known method. The obtained cuprocellulose solution was slowly stirred in the presence of air, and the degree of polymerization was adjusted over 12 hours. Furthermore, a coagulation solution having a tetrahydrofuran concentration of 89.00 wt % and a water concentration of 11.00 wt % was produced. While slowly stirring 5000 g of the coagulation liquid using a magnetic stirrer, 500 g of the cuprocellulose solution produced was added. After continuing stirring for about 5 seconds, 1000 g of 10 wt % sulfuric acid was added for neutralization and regeneration to obtain 6500 g of slurry containing cellulose fine particles.

[0165] The resulting slurry was centrifuged at 10000 rpm for 10 minutes. The precipitate was taken out by decantation, poured into distilled water, stirred, and centrifuged ...

Embodiment 2～6

[0180] The chromogenic particles were produced by the same method as in Example 1 except that the degree of polymerization described in the following Table 1 was adjusted to the cellulose fine particles, an immunochromatographic diagnostic kit was produced, and its performance was evaluated. The results are shown in Table 1 below.

Embodiment 7～9

[0182] Reactive dyes having a pyrimidine structure (LevafixRubineCAGR. (Example 7, registered trademark) manufactured by DyStar Ltd., LevafixNavyBlueE-BNACAGR. (Example 8, registered trademark) manufactured by DyStar Ltd., LevafixNavyCA GR manufactured by DyStar Ltd. (Example 8) were used. 9. Registered trademark)) The cellulose fine particles were dyed, and the same method as in Example 1 was used to prepare color-developing particles, and an immunochromatographic diagnostic kit was manufactured, and its performance was evaluated. The results are shown in Table 1 below.

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Abstract

The present invention provides organic colored microparticles used in an immunochromatography diagnostic kit, the microparticles having good background and test result reproducibility and sufficient detection sensitivity. These organic colored microparticles are characterized in that the average particle diameter is 100 to 650 nm; coloration intensity is 1.0 to 10.0; when a total of 100,000 particles are detected within a particle diameter range of 400 to 12,000 nm, the percentage of coarse particles having a particle diameter of 700 nm or greater is 5% or less; and the sphericity representedby the major axis (L) / minor axis (D) is 1.0 to 2.5.

Description

technical field [0001] The present invention relates to organic colored fine particles derived from organic polymers, a diagnostic kit and an in vitro diagnostic method using the same. Background technique [0002] In recent years, a simple test has been developed to perform various tests such as the presence or absence of pathogen infection such as viruses and bacteria, the presence or absence of pregnancy, the presence or absence of cancer markers, the presence or absence of specific raw materials in food, and the presence or absence of harmful substances such as residual pesticides in a short period of time. Reagents, diagnostic drugs, diagnostic kits. These utilize specific reactions based on each test substance and a substance that specifically reacts with the test substance. In particular, using immunological assays based on antigen-antibody reactions of antigens and antibodies, immunochromatographic assays, turbidimetric immunoassays, enzyme immunoassays, chemilumine...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/558G01N33/585G01N33/54313G01N33/54387G01N33/548G01N33/54388
Inventor 堀井厚志盐见祥之美村信之村冈谦
Owner ASAHI KASEI KK
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