High-throughput automatic continuous cell culture method and application thereof

Abstract

The invention discloses a high-throughput automatic continuous cell culture method and application thereof. The method comprises the following steps: (1) conducting inoculation and culture on a provided cell culture; (2) conducting cell sedimentation, and removing supernate in reactors; (3) adding a fresh culture medium; (4) calculating a release rate (B) according to the density of cells in the supernate, and maintaining the density of the cells; and (5) repeating the steps (2) to (4) until continuous cell culture is completed. According to the high-throughput automatic continuous cell culture method and application thereof, compared with a traditional perfusion reactor, a provided continuous cell culture model is high in throughput, high in automation degree, small in culture volume andlow in cost; and compared with culture by means of shaking, the high-throughput automatic continuous cell culture method has strong rector characteristics, strong culture result representation, low probability of personal errors and high reliability. Moreover, an additional cell retention device in the perfusion reactor does not need to be used in the high-throughput automatic continuous cell culture method, so that the problem that the cell retention device is liable to be blocked is solved.

Description

[0001] Priority and related applications [0002] This application claims the priority of the Chinese patent application 201811295263.4, filed on November 1, 2018, entitled High-throughput, automated continuous cell culture method and its application, the entire content of this application including the appendix is ​​incorporated by reference into this application . technical field [0003] The invention relates to the fields of biopharmaceuticals and biotechnology, in particular to the technical field of animal cell culture, and in particular to a high-throughput and automatic continuous cell culture method and its application in this field. Background technique [0004] After decades of development, cell culture technology has become a mature technology for the production of many important proteins (especially those glycosylated complex macromolecular proteins). These proteins are mostly used in the prevention and treatment of some serious human diseases, such as cancer, ...

AI Technical Summary

Problems solved by technology

But it also has its disadvantages, mainly as follows: 1) The perfusion reactor is equipped with a cell interception device, pumps / scales / pipelines for maintaining continuous culture, and complex control components, and its complex operability can easily cause human errors, And it takes a lot of manpower and time for daily operation and monitoring

2) Although the cell interception device has a good interception effect, in the case of long-term operation, the interception membrane pores are easily blocked, resulting in the interception of the target protein in the reactor, and the protein yield is low and the quality is reduced.

3) The scale of the perfusion reactor is usually large (ranging from 3L to 200L), and the culture time is long (ranging from 30 days to 120 days). Continuous culture needs to feed culture medium 1 to 2 times the culture volume of the reactor every day, and long-term culture The consumption of medium is huge, and the cost will increase significantly

[0009] Ambr15 TM It is a high-throughput automated micro-bioreactor culture system produced by Sartorius. Its original design is for high-throughput screening and process optimization of fed-batch experiments. Those skilled in the art do not think that it has continuous The function of cultured culture, and there is no record in the prior art that it has the function of continuous culture

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