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A silver nanocluster fluorescent probe and its preparation method and its application in detecting active oxygen species

A fluorescent probe, silver nanocluster technology, applied in the field of biochemistry, can solve the problem of inability to distinguish reactive oxygen species

Active Publication Date: 2021-07-23
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, most of the methods for detecting reactive oxygen species using fluorescein are based on redox reactions, which can effectively detect reactive oxygen species. However, this method cannot distinguish different types of reactive oxygen species.

Method used

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  • A silver nanocluster fluorescent probe and its preparation method and its application in detecting active oxygen species
  • A silver nanocluster fluorescent probe and its preparation method and its application in detecting active oxygen species
  • A silver nanocluster fluorescent probe and its preparation method and its application in detecting active oxygen species

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Embodiment Construction

[0037] The implementation of the present invention is described below through specific specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation modes, and various modifications or changes can be made to the details in this specification based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that, in the case of no conflict, the following embodiments and features in the embodiments can be combined with each other. It should also be understood that the terminology used in the embodiments of the present invention is for describing specific implementations, not for limiting the protection scope of the present invention. The test methods for which specific conditions are not indicated in the fo...

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Abstract

The invention discloses a silver nano-cluster fluorescent probe, a preparation method thereof and an application thereof in detecting active oxygen species, wherein the silver nano-cluster fluorescent probe includes protein and silver nano-cluster. The invention uses the probe to sensitively and effectively detect different types of reactive oxygen species in tissues or cells.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a silver nanocluster fluorescent probe, a preparation method thereof and an application thereof in detecting active oxygen species. Background technique [0002] Active oxygen includes hydrogen peroxide, superoxide radicals, hydroxyl groups, etc. The increase in the content of active oxygen will lead to severe oxidative stress and eventually cause molecular damage to DNA, proteins and lipids. However, at present, people have not thoroughly studied the specific effects and degrees of influence of different types of reactive oxygen species on cells. Therefore, it is of great significance to design a tool that can effectively detect specific types of reactive oxygen species, and provide a reference for exploring the mechanism of tumorigenesis and development. At present, most methods for detecting reactive oxygen species using fluorescein are based on redox reactio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C09K11/58C09K11/02B82Y20/00B82Y40/00
CPCB82Y20/00B82Y40/00C09K11/025C09K11/58G01N21/6428G01N21/6458G01N2021/6432G01N2021/6439
Inventor 陈炳地黄鑫陈欣
Owner TONGJI UNIV