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A kind of Saccharomyces coronis and method for separating and purifying diketopiperazine dimer therefrom

A technology of Rhizoctonia coronoidans and Rhizoctonia spores is applied in the field of biomedicine, which can solve the problem of no literature report on the preparation method of diketopiperazine dimer, and achieve high industrial application value, high biological activity, and purification process. Simple and cost-effective results

Active Publication Date: 2022-03-18
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The research in our laboratory found that: Diketopiperazine dimer can also be produced by P. There is no literature report on the preparation method of ketopiperazine dimer

Method used

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  • A kind of Saccharomyces coronis and method for separating and purifying diketopiperazine dimer therefrom
  • A kind of Saccharomyces coronis and method for separating and purifying diketopiperazine dimer therefrom
  • A kind of Saccharomyces coronis and method for separating and purifying diketopiperazine dimer therefrom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Screening and Identification of Coronoid Saccharomyces coronoides Strains

[0044] Screening of strains:

[0045] Step 1. Enrichment: Grind 10g of Fuzhuan brick tea into powder, make a suspension with physiological saline, and use the dilution coating plate method to dilute the above suspension 10 times, and spread it on a plate, and pick the colony The large and bright yellow colonies are spores of Cystis coronis;

[0046] Step 2, primary screening and purification: make spore suspension from the picked spores of S. coronis, and the spore viability is 1×10 7 cfu / mL, take 800 μL and inoculate it on a PDA plate for culture, culture it in a constant temperature incubator at 28°C for 5 days, and collect golden yellow spores;

[0047]Step 3, re-screening: Streak the spores obtained in the previous step on a plate containing PDA to continue screening for 8 generations, culture on a PDA slant, and store at 4°C.

[0048] Identification of strains:

[0049] (1) Mo...

Embodiment 2

[0061] Example 2 Isolation and Purification of Diketopiperazine Dimer from Trichomonas coronoides

[0062] Step 1. Inoculate S. coronoidis on the PDA plate, cultivate for 5 days, and collect golden yellow S. coronis spores; make spore suspension from the selected S. coronis spores, and the spore activity is 1* 10 7 cfu / mL, take 800 μ L and inoculate it on a PDA plate, and collect it after 6 days to obtain the spores of Saccharomyces coronoides;

[0063] Step 2, get 50g of the Saccharomyces coronoidis spores collected in step 1 in a 1 liter beaker, and add 1000mL of 50% ethanol water extract by volume percentage as 1:20 (w / v) according to the ratio of material to liquid, Ultrasonic treatment for 15 minutes, repeated extraction 3 times to obtain spore extract;

[0064] Step 3, absorb D-101 (also can adopt S-8, AB-8, X-5, NRR or D-3520 to replace) macroporous adsorption resin on the spore extract solution described in step 2, then use 30% 5 column volumes were eluted with etha...

Embodiment 3

[0069] Example 3 Isolation and Purification of Diketopiperazine Dimer from Trichomonas coronoides

[0070] Step 1. Inoculate S. coronoidis on a PDA plate, culture for 7 days, and collect golden yellow S. coronis spores; make spore suspension from the picked S. coronis spores, and the spore viability is 1* 10 7 cfu / mL, take 800 μ L and inoculate it on the PDA plate, and collect it after 5 days to obtain the spores of Saccharomyces coronoides;

[0071] Step 2, get 50g of the Saccharomyces coronoidis spores collected in step 1 in a 1 liter beaker, add 1000mL of ethanol water extract with a volume percentage of 60% according to the ratio of solid to liquid at 1:20 (w / v), Ultrasonic treatment for 20 minutes, repeated extraction 3 times to obtain spore extract;

[0072] Step 3, absorb D-101 (also can adopt S-8, AB-8, X-5, NRR or D-3520 to replace) macroporous adsorption resin on the spore extract solution described in step 2, then with 40% 5 column volumes were eluted with ethano...

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Abstract

The invention discloses Eurotium cristatum XD-05, whose preservation number is CGMCC No.19024. In addition, the invention also discloses a method for isolating and purifying diketopiperazine dimer from Cystis coronaris. The S. coronoidis strain of the present invention is derived from Fuzhuan tea, and the strain is ecologically safe. For the first time, diketopiperazine dimer is isolated and purified from S. coronis derived from Fuzhuan tea. The separation and purification method is simple and efficient, and the separation The purified diketopiperazine dimer has a purity of over 98%.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a coronoid and a method for separating and purifying a diketopiperazine dimer therefrom. Background technique [0002] Fuzhuan tea has a long history in our country, from the original pure handmade tea to modern machine-made tea, from the "official tea" that was only available to special groups in the Qing Dynasty to the current border tea, from the original specialty that can only be produced in Shaanxi Tea can now be produced all over the country, but Fuzhuan tea is made through the "fahua" process, which has never changed. Therefore, Fuzhuan tea is unique among all kinds of tea drinks. Fu brick tea can not only supplement some special substances needed by the human body, but also reduce blood fat and blood sugar, and have the effect of losing weight. Studies have shown that: the dominant microorganism in Fuzhuan brick tea is the coronoid spp., and the "golden ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N3/00C12P17/18C07D519/00C12R1/645
CPCC12N1/14C12N3/00C12P17/182C07D519/00C12R2001/645C12N1/145
Inventor 范代娣朱晨辉刘刚惠俊峰张慧
Owner NORTHWEST UNIV
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