A Recombinant Corynebacterium blunt-tooth Overexpressing the Pii Signal Transduction Protein

Abstract

The invention discloses a recombinant coryneform bacillus overexpressing PII signal transduction protein, which belongs to the field of biotechnology. The present invention provides a recombinant Corynebacterium bacillus SYPA5-5 / pXMJ19-glnK capable of high production of L-arginine, which is inoculated into a fermentation medium and fermented for 96 hours. That is to say, the production of L-arginine in the fermentation broth can be as high as 49.53g / L, which is 28.65% higher than that of the wild-type Corynebacterium blunt-toothed SYPA5-5.

Description

technical field [0001] The invention relates to a recombinant Corynebacterium blunterforms overexpressing a PII signal transduction protein, and belongs to the field of biotechnology. Background technique [0002] L-arginine is a semi-essential amino acid in humans and animals, and has a variety of unique physiological and pharmacological effects. For example, studies have shown that L-arginine not only has a significant therapeutic effect on ammonia-toxic hepatic coma and viral hepatitis, but also prevents excessive accumulation of ammonia and causes poisoning (for details, see references: Yu Xiangpeng, Liu Jing, Liu Jie). , et al. Research progress on L-arginine synthesis, breeding and fermentation of high-yielding bacteria [J]. Journal of Microbiology, 2012(01):76-80.). Studies have shown that nitric oxide produced in the L-arginine-nitric oxide pathway can regulate a series of immune processes and play an all-round protective effect on the body (for details, see referen...

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Problems solved by technology

[0004] However, the existing microbial fermentation method still has certain defects, wherein the low yield is one of the defects that limit the industrial production process of L-arginine. For example, Mireille Ginesy et al. inoculated E. coli SJB009 into the fermentation medium to ferment 48h, only the output of L-arginine in the fermentation broth can reach 11.6g / L (see references for details: Ginesy M, Belotserkovsky J, Enman J, et al. Metabolic engineering of Escherichia coli for enhanced arginine biosynthesis[J]. Microb Cell Fact, 2015, 14(29).); Zhang Bin et al. inoculated Corynebacterium blunt-toothed MT-M4ΔproB into the fermentation medium and fermented for 108 hours, only the production of L-arginine in the fermentation broth could reach 16.5g / L (see references for details: Zhang B, Wan F, Qiu Y, et al.Increased L-arginineProduction by Site-directed Mutagenesis of N-acetyl-L-glutamate Kinase andpro B Gene Deletion in Corynebacterium crenatum.[J]. Biomedical and Environmental Sciences, 2015,28(12):864-87.); Xu M et al inoculated Corynebacterium bacillus SYPA5-5 overexpressing the lysE gene into the fermentation medium and fermented for 96h, only L -The output of arginine reaches 35.9g / L (see references for details: Xu M, Rao Z, Yang J, et al. The effect of a LYSE exporteroverexpression on L-arginine production in Corynebacterium crenatum[J].Current microbiology, 2013,67(3):271-8.)

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