Methods and compositions for the preservation of tissue

A tissue and cryopreservation technology, applied in the field of tissue, can solve the problems of overall tissue shrinkage of the cytoskeleton, loss of extracellular space, loss, etc.

Inactive Publication Date: 2020-07-03
FORM 62 LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Additionally, preservation using the ASC method can lead to loss of the extracellular space, loss of anchored neurotransmitter vesicles, unraveling of myelin, reorganization of the cytoskeleton, and overall tissue shrinkage

Method used

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  • Methods and compositions for the preservation of tissue
  • Methods and compositions for the preservation of tissue
  • Methods and compositions for the preservation of tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0155] Embodiment 1. A method of preserving tissue of a subject comprising:

[0156] washing the tissue by perfusing the tissue with a wash solution comprising an aqueous solution;

[0157] fixing the tissue by perfusing the tissue with a fixative solution comprising aldehyde; and

[0158] cryopreserving said tissue by perfusing said tissue with a cryoprotectant solution comprising a vitrifying agent;

[0159] Wherein said washing solution, said fixative solution or said cryoprotectant solution comprises dye or contrast agent.

Embodiment approach 2

[0160] Embodiment 2. A method of preserving a subject's tissue comprising:

[0161] washing the tissue by perfusing the tissue with a wash solution comprising an aqueous solution;

[0162] fixing the tissue by perfusing the tissue with a fixative solution comprising aldehyde; and

[0163] The tissue is cryopreserved by perfusing the tissue with a cryoprotectant solution comprising a vitrifying agent, the cryoprotectant solution having a vitrification temperature of about -80°C or higher.

Embodiment approach 3

[0164] Embodiment 3. A method of preserving tissue of a subject comprising:

[0165] By perfusing the washing solution with any one or more of (1) an aqueous solution and (2) an ion channel blocker, a calcium chelator, a thrombolytic agent, an antiplatelet drug, a respiratory poison, or a synaptic poison tissue to wash the tissue;

[0166] fixing the tissue by perfusing the tissue with a fixative solution comprising aldehyde; and

[0167] The tissue is cryopreserved by perfusing the tissue with a cryoprotectant solution that includes a vitrifying agent.

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Abstract

Described herein are methods of preserving a tissue of a subject, such as a brain or a portion thereof. The methods include washing the tissue by perfusing the tissue with a wash fluid comprising an aqueous solution; fixing the tissue by perfusing the tissue with a fixation fluid comprising an aldehyde; and cryoprotecting the tissue by perfusing the tissue with a cryoprotection fluid comprising avitrification agent. The wash fluid, the fixation fluid, and / or the cryoprotection fluid can include a dye or a contrast agent to monitor perfusion of the fluid through the tissue. In certain embodiments, the cryoprotection fluid has a vitrification temperature of about -80 DEG C or higher. The wash fluid can further include one or more of an ion channel blocker, a calcium chelator, a thrombolyticagent, an anti-platelet, a respiratory poison, or a synaptic poison. Also described herein are methods of analyzing the preserved tissue.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of priority to U.S. Provisional Patent Application No. 62 / 550,945, filed August 28, 2017, the entire contents of which are incorporated herein by reference. technical field [0003] The invention described herein relates to methods and compositions for the preservation of tissue, particularly brain tissue. Background technique [0004] The ability to fix and preserve tissue to maintain structure is important for maintaining clinical and scientific tissue samples in long-term studies. Including neural tissue from whole brain banking from human and non-human (such as rodent) sources is particularly important for studying many brain diseases such as Alzheimer's disease. See, eg, Samarasekera et al., Brain banking for neurological disorders, Lancet Neurology, Vol. 12, No. 11, pp. 1096-1105 (2013). [0005] Unfortunately, brain bank technology hasn't changed much since the 1960s. Human...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00A01N1/02
CPCA01N1/00A01N1/0226A01N1/0221
Inventor R·麦金太尔M·麦卡纳
Owner FORM 62 LLC
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