The invention provides disinfectant for skin disinfection. The disinfectant is prepared from rhizoma coptidis, cortex phellodendri, herba houttuyniae, herba violae, isatis root, wild chrysanthemum flower, herba taraxaci, semen momordicae, golden larch bark, apiary, garlic, borax, calamine, hedgehog hide, cortex dictamni, fructus cnidii, common carpesium fruit, fructus ulmi, spina gleditsiae, cutch and unprocessed liquorice root all of which are Chinese herbal medicine ingredients for skin disinfection, propylene glycol, deionized water, absolute ethyl alcohol, nonyl phenol polyoxyethylene ether and lauryl sodium sulfate. The skin disinfectant has a powerful bactericidal effect, basically has no stimulation or toxic effect on the skin, is stable in property, difficult to volatilize, free of offensive odor and worthy of popularization and application, can not produce drug resistance and makes up the defects of existing disinfectant.