Strain of rhizopus stolonifer FL-3 and application thereof in extracting pachymaran

A technology of Rhizopus stolonifera and FL-3 is applied in the application field of microbial fermentation technology in the extraction of tuckahoe polysaccharide, which can solve the problems such as large difference in polysaccharide yield, and achieve the effects of improving extraction yield and promoting dissociation.

Active Publication Date: 2020-07-10
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Different extraction processes have their own advantages and dis

Method used

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  • Strain of rhizopus stolonifer FL-3 and application thereof in extracting pachymaran
  • Strain of rhizopus stolonifer FL-3 and application thereof in extracting pachymaran
  • Strain of rhizopus stolonifer FL-3 and application thereof in extracting pachymaran

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Screening of strain FL-3

[0026] (1) Add about 10 g of Poria cocos powder that has been crushed through a 60-mesh sieve into a 250-mL Erlenmeyer flask, then add 20 mL of sterile water to moisten it, and incubate at a constant temperature of 28°C for 4 days. Dilute the enriched culture covered with mold with sterile water 1 × 10 6 After doubling, spread it on the PDA plate medium, culture at 28°C for 2 days, pick out mold colonies with different colors and shapes, transfer them to fresh PDA medium, place them in 28°C for 3 days, and obtain 6 purely cultured strains, respectively Numbered FL-1~FL-6. Add 5 mL of sterile water to the fresh plate cultures of the 6 strains, stir with an inoculation loop to suspend the spores, transfer the spore liquid to a sterile test tube, and adjust the spores with sterile water. The concentration is 1×10 8 individual / mL.

[0027] (2) 250-mL triangular flask is sterilized by dry heat at 160°C for 2 hours, add 10 g of Poria cocos pow...

Embodiment 2

[0040] Rhizopus stoloniferus FL-3 is applied to the extraction of polysaccharides, and can be operated according to the following steps:

[0041] (1) Preparation of spore liquid of Rhizopus stoloniferus FL-3: cryopreserved Rhizopus stoloniferus FL-3 (freeze-dried tube or plate colony) was inoculated on fresh PDA plate medium, cultured at 28°C for 3 days, and added 5 mL Put sterile water in a petri dish, stir with an inoculation loop to suspend the spores, transfer the spore liquid to a sterile test tube, and adjust the spore concentration to 1×10 with sterile water. 8 individual / mL. Described PDA plate medium composition and preparation method are the same as embodiment 1.

[0042] (2) 500-mL Erlenmeyer flask was sterilized by dry heat at 160°C for 2 hours, added 20 g of Poria cocos powder crushed through a 60-mesh sieve, added 60 mL of sterile water, and then inoculated with 2 mL of Rhizopus stoloniferus FL- 3 spore liquid, stir well. The mouth of the triangular flask was ...

Embodiment 3

[0047] Rhizopus stoloniferus FL-3 is applied to the extraction of polysaccharides, and can be operated according to the following steps:

[0048] (1) Preparation of spore liquid of Rhizopus stoloniferus FL-3: cryopreserved Rhizopus stoloniferus FL-3 (freeze-dried tube or plate colony) was inoculated on fresh PDA plate medium, cultured at 28°C for 3 days, and added 5 mL Put sterile water in a petri dish, stir with an inoculation loop to suspend the spores, transfer the spore liquid to a sterile test tube, and adjust the spore concentration to 2×10 with sterile water 8 individual / mL. Described PDA plate medium composition and preparation method are the same as embodiment 1.

[0049] (2) 2-L Erlenmeyer flask was sterilized by dry heat at 160°C for 2 hours, added 50 g of Poria cocos powder crushed through a 60-mesh sieve, added 150 mL of sterile water, and then inoculated with 5 mL of the spore liquid prepared in step (1) of FL-3 , stir well. The mouth of the triangular flask w...

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Abstract

The invention discloses a strain of rhizopus stolonifer FL-3 and application thereof in extracting pachymaran. The application method comprises the following steps: inoculating rhizopus stolonifer FL-3 spores into poria cocos powder containing sterile water, fermenting at 28-30 DEG C for 2-3 days, and concentrating the fermented product after ultrasonic water extraction to obtain a concentrate; and precipitating the concentrate and performing vacuum drying to obtain the pachymaran. In advance of ultrasonic water extraction of the pachymaran, fermentation pretreatment of the rhizopus stoloniferFL-3 is added. The rhizopus stolonifer FL-3 is separated from a microbial enrichment culture of poria cocos, and an excellent strain obtained by screening moderately grows in the poria cocos powder to generate polysaccharide hydrolase, so that hydrolysis of insoluble polysaccharide structural tissues of the poria cocos promotes dissociation of combined soluble polysaccharide; and compared with aconventional method without fermentation pretreatment, the extraction yield of the pachymaran can be improved by over 30%.

Description

[0001] (1) Technical field [0002] The invention belongs to the technical field of biochemical industry, and in particular relates to the application of a microbial fermentation technology in the extraction of pachyrhiza. [0003] (2) Background technology [0004] The traditional Chinese medicine Poria (Poria), also known as Poria rabbit and Songbaiyu, is the dried sclerotia of the fungus Poria cocos Wolf in the family Polyporaceae. Poria cocos is a traditional Chinese herbal medicine for both medicine and food. It is recorded in the 2015 Chinese Pharmacopoeia. Its nature and flavor are sweet, light and flat; Cure mainly edema, oliguria, dizziness and palpitation due to phlegm retention, insufficiency of the spleen, lack of food, loose stool and diarrhea, restlessness, palpitation and insomnia. Poria cocos is one of the components of the traditional Chinese medicine "Four Monarchs and Eight Treasures". It has a wide range of uses and a large dosage. There are hundreds of Chi...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/04C08B37/00C12R1/845
CPCC12P19/04C08B37/0003C12R2001/845C12N1/145
Inventor 梅建凤郑素晶吴霞应国清易喻
Owner ZHEJIANG UNIV OF TECH
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