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A method for identifying salmon species using characteristic identification peptides

A salmon and species technology, applied to the combination of characteristic peptides of salmon and rainbow trout, to detect salmon species (Atlantic salmon field, which can solve the problem of distinguishing different species of salmon)

Active Publication Date: 2022-08-05
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no method to accurately distinguish different species of salmon using mass spectrometry at home and abroad.

Method used

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  • A method for identifying salmon species using characteristic identification peptides
  • A method for identifying salmon species using characteristic identification peptides
  • A method for identifying salmon species using characteristic identification peptides

Examples

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Embodiment 1

[0040] Example 1: Obtaining specific characteristic peptides of 3 different salmon species

[0041] This application uses liquid mass analysis platforms (UHPLC-HRMS and UHPLC-MS / MS) to carry out protein / peptidomics analysis of salmon from three different species of Atlantic salmon, salmon and rainbow trout, combined with bioinformatics methods. The generated mass spectrometry data were mined and analyzed, and 7 identifying signature peptides (ISPs) were screened out for the identification of three common salmon species in the market.

[0042]1. Protein / peptide identification and analysis based on IDA data:

[0043] Through protein retrieval, a total of 140,295 pieces of protein information from three species of Atlantic salmon, salmon and rainbow trout were obtained. Among them, Atlantic salmon ( Salmo salar ) is currently the most informative species in "Salmo" (Salmo) in the Uniprot proteome database, with as many as 89,081, while salmon ( Oncorhynchus keta ) information...

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PUM

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Abstract

The invention discloses a method for identifying salmon species by using characteristic identification peptides. The characteristic identification peptide segment, that is, the polypeptide marker, includes the unique characteristic peptide segment of Atlantic salmon, salmon and rainbow trout, wherein the characteristic peptide amino acid sequence of Atlantic salmon is AADTFNFK, YIIESVGNIR, AAVPSGASTGIHEALELR and NGLMIAEIEELR, and salmon features The amino acid sequence of the peptide is LLATLYPAAPPEDK, and the characteristic peptide sequences of rainbow trout are AADSFNFK and ISALDLSGDQAALMEMLK. The invention realizes the identification of common salmon species by detecting the specific characteristic polypeptides found by high-performance liquid chromatography-mass spectrometry, and provides a certain basis for accurately distinguishing Atlantic salmon and its closely related species, salmon and rainbow trout. Theoretical basis and technical support.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a combination of characteristic peptides and a method for detecting salmon species (Atlantic salmon, salmon and rainbow trout). Background technique [0002] Salmon is a general term for salmonids, belonging to large and medium-sized cold water migratory fish, mainly distributed in the northern Pacific Ocean and the border waters of the Atlantic Ocean and the Arctic Ocean, including 3 subfamilies, 7 genera and about 67 species of fish. Common red meat salmon on the market include Atlantic salmon ( Salmo salar ), salmon ( Oncorhynchus keta ) and rainbow trout ( Oncorhynchus mykiss ) and so on, among which Atlantic salmon has delicate muscle tissue, delicious meat and rich in protein and fatty acids. Compared with Atlantic salmon, freshwater rainbow trout and salmon of the Pacific salmon genus have slightly rough meat, and are mostly used for processing into...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06
CPCG01N30/06G01N30/72G01N30/88
Inventor 陈颖张九凯马聪聪韩建勋邢冉冉
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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