Drug for treating cerebral apoplexy and construction method of endogenous stem cell expression animal model

Abstract

The invention belongs to the technical field of medicine and pharmacology, and discloses a drug for treating cerebral apoplexy and a construction method of an endogenous stem cell expression animal model, and the drug for treating cerebral apoplexy is liver-softening collateral-dredging decoction. The number of rat brain tissue BrdU, BrdU / NeuN and BrdU / GFAP positive cells in each group at each time point is detected through an ischemia reperfusion model caused by middle cerebral artery occlusion, and the influence of the liver softening and collateral dredging soup on proliferation and differentiation of endogenous neural stem cells of rats with ischemic brain injury is analyzed. According to the method, an improved Longa method is adopted to manufacture an ischemia-reperfusion brain injury model caused by MCAO type middle cerebral artery occlusion of rats; it is verified that the liver-softening collateral-dredging decoction promotes proliferation of endogenous neural stem cells of ischemia-reperfusion brain injury rats, differentiates the endogenous neural stem cells into mature neurons, inhibits excessive proliferation of astrocytes, promotes nerve regeneration and repair and improves nerve functions.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a drug for treating cerebral apoplexy and a method for constructing an animal model expressing endogenous stem cells. Background technique [0002] At present, cerebrovascular disease is one of the three major diseases leading to the death of the global population, seriously endangering the daily activities of middle-aged and elderly people, and its high treatment and rehabilitation costs bring a heavy burden to families and society. Among them, ischemic cerebrovascular disease (ICD) accounts for about 60% to 80% of all cerebrovascular diseases. After ischemic stroke, the blood supply of brain tissue is hindered, and neurons become inflammatory, edema, denatured and even necrotic due to ischemia and hypoxia, which eventually leads to damage or even loss of information transmission function and integration function in the central nervous system. Endogenous neural ste...

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Problems solved by technology

[0003] Through the above analysis, the existing problems and defects of the prior art are: in the analysis of the influence of the prior art on the proliferation and differentiation of endogenous neural stem cells in ischemic brain injury, the evidence provided is poor; Its potential role in the treatment of stroke provides an effective theoretical basis

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