Method for simultaneously determining ethanol non-oxidative metabolites in blood and vitreous humor
A vitreous humor, non-oxidative technology, applied in the fields of analytical chemistry and forensic identification, can solve the problems of ethanol non-oxidative metabolites, and achieve the effects of high sensitivity, easy operation, and less sample consumption
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[0028] The chromatographic conditions are as follows:
[0029] Chromatographic column: Phenomenex Synergi 2.5μm Hydro-RP 100A column (2mm×100mm, 2.5μm); column temperature: 40℃
[0030] Mobile phase: water (0.1% formic acid) (phase A); acetonitrile (0.1% formic acid) (phase B); gradient elution (see Table 3), flow rate 0.25mL / min.
[0031] Injection volume: 5μL
[0032] Table 3 gradient elution conditions
[0033]
[0034] The mass spectrometry conditions are as follows:
[0035] ESI; spray voltage: 3.6kV(+) / 2.8kV(-); sheath gas: 35Arb; auxiliary gas: 10Arb; ion transfer tube temperature: 350°C; desolvation temperature: 300°C;
[0036] Scanning method: multiple reaction monitoring (MRM)
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