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Method for simultaneously determining ethanol non-oxidative metabolites in blood and vitreous humor

A vitreous humor, non-oxidative technology, applied in the fields of analytical chemistry and forensic identification, can solve the problems of ethanol non-oxidative metabolites, and achieve the effects of high sensitivity, easy operation, and less sample consumption

Inactive Publication Date: 2020-08-04
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the current state of the art, especially in the field of forensic identification, it is urgent to establish an analytical method that can be used for the simultaneous determination of EtG and EtS in blood and vitreous humor; It can solve the problem of detecting non-oxidative metabolites of ethanol in blood or vitreous humor alone, and will provide an accurate and reliable new method for judging whether the parties have ingested ethanol in judicial appraisal

Method used

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  • Method for simultaneously determining ethanol non-oxidative metabolites in blood and vitreous humor
  • Method for simultaneously determining ethanol non-oxidative metabolites in blood and vitreous humor
  • Method for simultaneously determining ethanol non-oxidative metabolites in blood and vitreous humor

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Embodiment 1

[0028] The chromatographic conditions are as follows:

[0029] Chromatographic column: Phenomenex Synergi 2.5μm Hydro-RP 100A column (2mm×100mm, 2.5μm); column temperature: 40℃

[0030] Mobile phase: water (0.1% formic acid) (phase A); acetonitrile (0.1% formic acid) (phase B); gradient elution (see Table 3), flow rate 0.25mL / min.

[0031] Injection volume: 5μL

[0032] Table 3 gradient elution conditions

[0033]

[0034] The mass spectrometry conditions are as follows:

[0035] ESI; spray voltage: 3.6kV(+) / 2.8kV(-); sheath gas: 35Arb; auxiliary gas: 10Arb; ion transfer tube temperature: 350°C; desolvation temperature: 300°C;

[0036] Scanning method: multiple reaction monitoring (MRM)

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Abstract

The invention belongs to the technical field of analytical chemistry and judicial expertise, relates to a method for determining ethanol non-oxidative metabolites in blood and vitreous humor, and particularly relates to a method for simultaneously determining two types of ethanol non-oxidative metabolites, namely, ethyl sulfate and ethyl glucuronate in blood and vitreous humor. The method has theadvantages of less detection material consumption, high sensitivity, strong specificity, wide linear range, simple and rapid operation, less extraction solvent consumption and short analysis time, canmeet the requirements of urgent judicial expertise tasks and high requirements on detection time, and can be popularized and applied in a large range. The method can be applied to two conventional samples of blood and vitreous humor at the same time, can provide results of EtG and EtS in the vitreous humor as a reference compared with pure blood, makes up for the problems existing in single sample determination, and improves the reliability of the detection result.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and forensic identification, and relates to a method for measuring ethanol non-oxidative metabolites in blood and vitreous humor. In particular, it relates to a method for simultaneously determining two types of ethanol non-oxidative metabolites in blood and vitreous humor, especially a simultaneous detection method for ethyl sulfate and ethyl glucuronate in human blood and vitreous humor. The method requires less material, high sensitivity, strong specificity, wide linear range, simple and fast operation, less extraction solvent, and short analysis time, which can meet the needs of urgent judicial appraisal tasks and high detection time requirements. It can be widely applied. Background technique [0002] The prior art discloses that ethyl glucuronate (EtG) and ethyl sulfate (EtS) are non-oxidative metabolites of ethanol, which are produced by phase II metabolism of ethanol, and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 饶渝兰王昊黄志斌樊恩杉
Owner FUDAN UNIV