A kind of detection method of p-hydroxyacetophenone related substances
A technology of p-hydroxyacetophenone and hydroxyacetophenone, which is applied in the field of drug analysis and detection, can solve problems such as unrecorded hydroxyacetophenone detection methods, and achieve the effects of improving drug safety, high sensitivity, and good reproducibility
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[0035] The embodiment of the present invention provides a kind of detection method of p-hydroxyacetophenone related substances
[0036] 1.1 Materials and methods
[0037] Instruments: gas chromatograph, hydrogen flame ionization detector, measuring flask, electronic balance.
[0038] Blank solvent: 99% methanol and 1% water.
[0039] 1.2 Preparation of the solution
[0040] Reference solution: take appropriate amounts of phenol, phenol acetate, 2'-hydroxyacetophenone and 3'-hydroxyacetophenone, accurately weigh them, and prepare each 1mL reference solution with 99% methanol and 1% water. Contains 50 µg of phenol, 50 µg of phenol acetate, 50 µg of 2'-hydroxyacetophenone, and 50 µg of 3'-hydroxyacetophenone.
[0041] Test solution: Take an appropriate amount of p-hydroxyacetophenone to be tested, accurately weigh it, and use 99% methanol and 1% water to prepare 10 mg of p-hydroxyacetophenone test solution per 1 mL of test solution.
[0042] 1.3 Detection methods of p-hydroxy...
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