A hemoglobin oxygen carrier based on double chemical modification and its preparation method and application

A technology of hemoglobin and chemical modification, applied in the field of biomedicine, can solve the problems of low colloidal osmotic pressure and cannot effectively expand blood volume, etc., and achieve the effects of increasing colloidal osmotic pressure, enhancing the activity of carrying/releasing oxygen, and improving stability.

Active Publication Date: 2022-05-13
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, because the intermolecular cross-linking mediated by glutaraldehyde reduces the number of colloidal particles of HbA, its colloid osmotic pressure is also lower than that of blood, and thus cannot effectively expand blood volume

Method used

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  • A hemoglobin oxygen carrier based on double chemical modification and its preparation method and application
  • A hemoglobin oxygen carrier based on double chemical modification and its preparation method and application
  • A hemoglobin oxygen carrier based on double chemical modification and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0063] Separation and purification of adult hemoglobin (HbA):

[0064] Centrifuge adult whole blood and discard the supernatant. Suspend with PBS buffer (pH=7.4), centrifuge and discard the supernatant. Repeat washing twice. Red blood cells were lysed with 3 volumes of water. Centrifuge the erythrocyte lysate, take the supernatant, and filter it with a 0.22 μm filter membrane. The filtrate was separated and purified by Q Sepharose Fast Flow anion exchange chromatography medium, and the elution peak corresponding to adult hemoglobin (HbA) was collected, concentrated, dialyzed into PBS buffer (pH=7.4), and stored at -80°C for future use.

Embodiment 2

[0066] Preparation of glyoxylated hemoglobin:

[0067] Glyoxylic acid covalently modifies the N-terminal Val-1(α) of HbA, thereby introducing a carboxymethyl group, which will destroy the salt bridge that participates in the formation of the stable R-state structure and reduce the stability of the relaxed state (R-state) of HbA , so that the HbA quaternary structure shifts from the R state to the tense state (T state), reducing the affinity of HbA for oxygen.

[0068] Deoxygenated HbA was prepared by continuously bubbling nitrogen gas into the 0.5 mM HbA solution. Then 1.0 mM glyoxylic acid and 10 mM sodium cyanoborohydride (NaCNBH 3 ) solution, so that the mol ratio of HbA, glyoxylic acid and sodium cyanoborohydride is 1:2:20. Continuing to feed nitrogen, the reaction was carried out at 4° C. for 3.5 h under anaerobic conditions to obtain glyoxylic acid-modified HbA (expressed as Glx-Hb). Finally, 20 mM glycine solution was added to terminate the reaction, and unreacted gl...

Embodiment 3

[0070] Preparation of DBBF intramolecularly cross-linked hemoglobin:

[0071] When bis(3,5-dibromosalicylic acid) fumarate (DBBF) modified deoxy HbA, an intramolecular cross-linking reaction occurred between two Lys-99(α).

[0072] Deoxygenated HbA was prepared by continuously bubbling nitrogen gas into the 0.5 mM HbA solution. Subsequently, an equal volume of 4 mM inositol hexaphosphate (IHP) solution was added, nitrogen gas was continuously introduced, and the reaction was carried out at 25° C. for 3 h under anaerobic conditions. Subsequently, 0.5 mM DBBF solution was added so that the molar ratio of HbA to DBBF was 1:1, and reacted at 25° C. for 4 h to obtain intramolecularly cross-linked HbA (expressed as αα-Hb) of DBBF. Finally, 20 mM glycine solution was added to terminate the cross-linking reaction, and unreacted DBBF and glycine were removed by dialysis. The schematic diagram of its preparation is as Figure 13 shown.

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Abstract

The present invention relates to a hemoglobin oxygen carrier based on double chemical modification and its preparation method and application. In the hemoglobin oxygen carrier based on double chemical modification, two Val‑1 (α) residues are carboxymethylated, and two Lys ‑99(α) residues are intramolecularly cross-linked. Two of the Lys-99(α) residues are intramolecularly cross-linked, and the fumarimide bridge formed can change the electrostatic interaction of adult hemoglobin (HbA) molecules, stabilize the tense state (T state) of HbA, and reduce its Oxygen affinity; Val‑1(α) is located at the αα‑end of the central cavity of HbA, and can participate in the formation of salt bridges that help stabilize the structure of the relaxed state (R state). Carboxylation modification will destroy these salt bridges and reduce HbA The stability of the R state makes the quaternary structure of HbA shift from the R state to the T state, reducing the affinity of HbA for oxygen. The invention organically combines the two modification means, improves the stability of the HbA tetramer, enhances the electrostatic interaction, improves the binding state of the oxygen binding region and oxygen, and has strong oxygen carrying / releasing activity.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a hemoglobin oxygen carrier and its preparation method and application, in particular to a hemoglobin oxygen carrier based on double chemical modification and its preparation method and application. Background technique [0002] In recent years, with the rapid growth of clinical blood demand in my country and the implementation of the voluntary blood donation system, the contradiction between blood supply and demand has become increasingly prominent. The traditional blood transfusion method plays an important role in medical rescue, but also has various shortcomings, mainly reflected in: (1) Due to the existence of the window period, blood transfusion has the risk of pathogen transmission, such as HIV, hepatitis B virus and other pathogens may exist in the blood ; (2) There is a shortage of blood sources in my country, and clinical blood banks have insufficient rese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/805C07K1/107C07K1/113C07K1/34C07K1/16A61K38/42A61P7/08
CPCC07K14/805A61P7/08
Inventor 胡涛颜文颖于卫立申莉娟
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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