7 results about "Oxygen binding" patented technology

The invention discloses ternary synergistic nano-enzyme hydrogel as well as a preparation method and application thereof, and relates to the technical field of biomedical materials. The preparation method comprises the following steps: dissolving zinc nitrate hexahydrate and ferric nitrate nonahydrate in methanol; dissolving 2-methylimidazole in methanol, adding into a metal salt solution, stirring, and sequentially centrifuging, washing and drying to obtain Fe / ZIF-8 nano-enzyme; the preparation method comprises the following steps: adding methacrylated gelatin and hyaluronic acid into deionized water, stirring, cooling to room temperature, and refrigerating to obtain a GelMA-HA precursor solution; and adding the Fe / ZIF-8 nano-enzyme into the GelMA-HA precursor solution, stirring, injecting into a mold, and carrying out ultraviolet light cross-linking curing to obtain the ternary synergistic nano-enzyme hydrogel. According to the hydrogel disclosed by the invention, active oxygen is efficiently removed and oxygen is produced through Fe / ZIF-8 nano-enzyme, and cartilage repair is promoted in combination with lubrication and slow release functions of gelatin hydrogel, so that a new strategy is provided for treatment of osteoarthritis.

The present invention describes chimeric proteins having albumin or truncated albumin covalently linked to one or more oxygen-binding proteins or protein fragments or variants, their preparation and use

The current disclosure relates to a method for determining the presence of a specific variant of a given protein, for example hemoglobin, capable of binding an analyte, for example oxygen, in cells of a biological sample, said method comprising determining the binding affinity of said analyte to said protein in a plurality of cells, for example red blood cells within said sample. The protein can be hemoglobin, which is capable of binding to oxygen, and the presence of specific variant of hemoglobin in cells of a blood sample is determined by the oxygen binding affinity of hemoglobin in a plurality of red blood cells within said sample. The method is also used for determining the presence of one or more specific types of red blood cells in a blood sample comprising analysing a blood sample using hyperspectral imaging. The method further relates to monitoring a disease associated with the presence of a specific variant of a given protein capable of binding an analyte in cells of an individual afflicted with said disease, wherein the method comprises the step of determining the presence of said specific variant of said protein in cells of a biological sample obtained from said individual. For example, the method is used for monitoring a disease associated with the presence of a specific variant of hemoglobin in red blood cells in an individual and / or in a fetus carried by said individual, wherein the method comprises determining the oxygen binding affinity of hemoglobin in a plurality of red blood cells in a blood sample from obtained from said individual. Moreover, the method is used for monitoring a disease relating to the presence of different types of red blood cells in an individual and / or in a fetus carried by said individual, wherein the method comprises the step of determining the presence of one or more specific types of red blood cells in a blood sample obtained from said individual by analysing said blood sample using hyperspectral imaging. The disclosure also relates to a measuring instrument configured to perform measurements following the disclosed methods.

Metastable alloys have recently emerged as high-performance catalysts, extending the toolbox of binary alloy materials that can be utilized to mediate electrocatalytic reactions. In particular, nanostructured metastable ordered intermetallic compounds are particularly challenging to synthesize. Here the present invention is directed to a method for synthesizing sub-15 nm metastable ordered intermetallic Pd31Bi12 nanoparticles at room temperature, in a single step, by pulsed electrochemical deposition onto high surface area carbon supports. The resulting Pd31Bi12 nanoparticles displays a 7× enhancement of the mass activity relative to Pt / C and a 4× enhancement relative to Pd / C for the oxygen reduction reaction (ORR). The high performance of Pd31Bi12 nanoparticles is demonstrated to arise from reduced oxygen binding caused by alloying of Pd with Bi. The isolation of Pd-sites from each other facilitate methanol tolerant ORR behavior.

This invention relates to an intelligent emergency respiratory support system and its driving method. The system includes: a first acquisition unit for collecting hemodynamic characteristic information of a patient; a second acquisition unit for collecting respiratory characteristic information of the patient during the time period of collecting hemodynamic characteristic information; an oxygen supply unit for providing oxygen to the patient based on the patient's hemodynamic and respiratory characteristic information; and a processor, which is signal-connected to the first acquisition unit, the second acquisition unit, and the oxygen supply unit. Based on the hemodynamic characteristic information, the processor obtains blood oxygen saturation, reflecting the degree of oxygen binding to hemoglobin in the patient's arterial blood, and blood flow velocity, reflecting the speed of blood flow in the patient's blood vessels. Simultaneously, based on the respiratory characteristic information, the processor obtains the patient's respiratory rate, and controls the oxygen supply unit to change the oxygen supply mode provided to the patient. This invention can solve the problem that existing technologies cannot effectively improve emergency rescue efficiency because they ignore the patient's actual oxygen consumption characteristics.

The disclosure relates to a system and method for assessing red blood cell oxygen affinity involving spectrophotometric oxygen dissociation assay (SODA).

The invention relates to an engineered cell for heterologous co-expression in escherichia coli and a construction method of the engineered cell, and belongs to the technical field of microorganisms. The construction method comprises the following steps: (1) constructing a fusion gene; (2) performing escherichia coli preference codon optimization; (3) constructing a recombinant plasmid pET-28a-EHb and a recombinant plasmid pET-28a-EMb, and constructing a recombinant plasmid pET-28a-EHb; (4) carrying out PCR (Polymerase Chain Reaction) amplification to obtain an HbF fusion gene segment and an Mb gene segment; (5) constructing a co-expression fragment through fusion PCR; (6) constructing a co-expression recombinant plasmid; and (7) transforming the co-expression recombinant plasmid into an escherichia coli host cell, and culturing to obtain an engineered cell for heterologous co-expression in escherichia coli. The invention constructs a co-expression system based on fetal hemoglobin and fetal myoglobin, and obtains a recombinant heme protein composition with oxygen binding performance, molecular stability and high expression efficiency.