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41 results about "Oxygen binding" patented technology

Oxygen binding capacity is the maximum amount of oxygen that can be bound to hemoglobin, abbreviated as Hb, which is the main protein found inside of red blood cells, which is a main component of blood. As it turns out, there are two major ways for oxygen to move around in the blood.

Method for recombining Saccharopolyspora erythraea strain containing exogenetic vitreoscilla hemoglobin gene (vgb)

InactiveCN102234654AUltimate titer increaseUltimate cost-effectivenessFungiMicrobiological testing/measurementBinding stateFermentation
The invention belongs to the technical field of gene engineering, and provides a method for recombining a Saccharopolyspora erythraea strain containing exogenetic vitreoscilla hemoglobin gene (vgb). In the method, vitreoscilla hemoglobin (VHb) expressed by the vitreoscilla hemoglobin gene (vgb) can be combined with oxygen to form an oxygen-binding state, and intervenes several key steps or branch pints in cell metabolic paths related with oxygen in the mode, so that the cell growth speed is improved, the cell respiration intensity is improved and the critical oxygen concentration of cells is reduced. In large dissolved oxygen change, a constant respiratory rate is kept, and the cell culture density and exogenetic gene expression are improved under low oxygen condition. The method solves the problem that in a large-scale bioreactor, because thalli are positioned in a viscous, enclosed and complicated environment with rich nutrients, oxygen required by massive mycelia in an exponential phase is limited, and the yield of erythrocin is reduced. The method has important application value for the antibiotic fermentation industry and high density cell culture, which have high oxygen consumption and take dissolved oxygen as a limiting factor.
Owner:邢安辉 +2

An energy-saving combined beneficiation process for high-oxidation, high-combined oxygen-sulfur mixed copper ore

The invention provides an energy-saving combined ore dressing process for high-oxidation and high-bonding oxygen and sulfur mixed copper ore. The process comprises the steps of raw ore treatment, preparation of leaching solution, stirring and leaching, dynamic soaking selection, solid-liquid separation and washing and filtration of leached residue. The ore is crushed till the granularity is less than 10 millimeters, and the crushed ore with granularity of less than 3 millimeters is screened; sulfuric acid solution, the PH of which is less than 8, is used as the leaching solution; the raw ore is dynamically and continuously soaked for less than 24 hours, and the leachate is directly guided to an extraction process after PH value neutralization and micro pipe filtration; and the leached residue is separated by using a belt filter, and the filtered residue is treated in a flotation process to extract copper sulfide ore. According to the combined efficient washing and filtering process, dynamic soaking is adopted, the leachate is subjected to micro pipe filtration (the leachate with solid concentration of less than or equal to 3 percent in liquid can be filtered, and the solid content of the filtered liquid can be less than 50ppm), and the leached residue is separated by using the belt filter, so that the ore can meet the process requirement without fine crushing. By the process, the metal recovery rate of the high-bonding oxidized copper ore reaches over 80 percent, continuous production is realized, and energy consumption and production cost are comprehensively reduced.
Owner:云南楚雄矿冶有限公司

Biodegradable Nanoparticles as Novel Hemoglobin-Based Oxygen Carriers and Methods of Using the Same

ActiveUS20140335160A1Low oxygenation of tissueLow oxygenationBiocidePeptide/protein ingredientsEthylene oxideBlood substitute
Compositions of matter and methods for making, storing and administering artificial blood substitutes. Artificial blood substitutes may have oxygen carriers that encapsulate an oxygen-binding compound in a polymer vesicle. Oxygen-binding compounds may include hemoglobin, myoglobin, or other oxygen binding compounds having characteristics similar to hemoglobin. Oxygen carriers may include nanoparticles, polymers and / or polymersomes comprising of poly(ethylene oxide)-block-poly(ε-caprolactone) (PEO-b-PCL) and related diblock copolymers of poly(ethylene oxide)-block-poly(γ-methyl ε-caprolactone) (PEO-b-PMCL). The oxygen carriers may have tunable oxygen-binding capacities, uniform and appropriately small size distributions, and human bloodlike viscosities and oncotic properties.
Owner:POSEIDA THERAPEUTICS INC

A hemoglobin oxygen carrier based on double chemical modification and its preparation method and application

ActiveCN111499732BImprove oxygen carrying/releasing activityEnhanced oxygen-carrying/evolving activityPeptide/protein ingredientsHaemoglobins/myoglobinsCarboxyl radicalBiophysics
The present invention relates to a hemoglobin oxygen carrier based on double chemical modification and its preparation method and application. In the hemoglobin oxygen carrier based on double chemical modification, two Val‑1 (α) residues are carboxymethylated, and two Lys ‑99(α) residues are intramolecularly cross-linked. Two of the Lys-99(α) residues are intramolecularly cross-linked, and the fumarimide bridge formed can change the electrostatic interaction of adult hemoglobin (HbA) molecules, stabilize the tense state (T state) of HbA, and reduce its Oxygen affinity; Val‑1(α) is located at the αα‑end of the central cavity of HbA, and can participate in the formation of salt bridges that help stabilize the structure of the relaxed state (R state). Carboxylation modification will destroy these salt bridges and reduce HbA The stability of the R state makes the quaternary structure of HbA shift from the R state to the T state, reducing the affinity of HbA for oxygen. The invention organically combines the two modification means, improves the stability of the HbA tetramer, enhances the electrostatic interaction, improves the binding state of the oxygen binding region and oxygen, and has strong oxygen carrying / releasing activity.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI
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