A kind of nano enzyme, preparation method and application thereof

A nano-enzyme and enzyme-mimicking technology, which is applied in the field of nano-enzyme catalysis, can solve the problems of graphyne research and achieve the effects of more active sites, improved catalytic activity, and high activity

Active Publication Date: 2021-09-28
北京高能科迪科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research on graphyne in imitating enzyme catalysis.

Method used

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  • A kind of nano enzyme, preparation method and application thereof
  • A kind of nano enzyme, preparation method and application thereof
  • A kind of nano enzyme, preparation method and application thereof

Examples

Experimental program
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preparation example Construction

[0040] It should be noted that the present invention also provides a method for preparing nitrogen-doped graphyne nanozyme, comprising the following steps:

[0041] Step S1, preparing graphyne oxide: using an oxidizing agent to oxidize the original graphyne to obtain graphyne oxide;

[0042] Step S2, annealing graphyne oxide in a nitrogen source to obtain nitrogen-doped graphyne nanozyme.

[0043] Specifically, in the above step S1, the oxidizing agent is strong acid, potassium permanganate or H 2 o 2 . In order to avoid introducing metal impurities, preferably, the oxidizing agent is a strong acid.

[0044] Specifically, in the above step S1, the step of using a strong acid as an oxidizing agent to oxidize the original graphyne includes:

[0045] S11. Mix the original graphyne with a strong acid, heat to 70-90°C (the temperature is too low, the reaction rate is slow; the temperature is too high, the strong acid is easy to decompose and volatilize), and keep warm for 2.5-3...

Embodiment 1

[0064] This embodiment provides a graphyne oxide, the preparation method is as follows:

[0065] Mix 40 mg of raw graphyne with 40 mL of concentrated HNO 3 / Concentrated H 2 SO 4 (1:3) mixed, reacted at 80°C for 3 hours, cooled to room temperature, added water and centrifuged several times, and when the pH of the supernatant was >6, the lower layer samples were collected and freeze-dried to obtain graphyne oxide (GDYO).

[0066] Among them, raw graphdiyne (GDY) is purchased from the conventional market. Such as figure 1 as shown, figure 1 It is a comparison chart of the dispersion of original graphyne and graphyne oxide; (a)-(c), the right side is original graphyne, the left side is graphyne oxide, (a) is the dispersion of original graphyne and graphyne oxide Photos after ultrasonication in water; (b) is the photo of pristine graphyne and graphyne oxide dispersed in water after ultrasonication for 10 min; (c) is the photo of original graphyne and graphyne oxide dispersed...

Embodiment 2

[0068] The present embodiment provides a nitrogen-doped graphyne nanozyme, wherein the nitrogen atomic percentage is 9.79% (such as figure 2 shown).

[0069] This embodiment also provides a nitrogen-doped graphyne nanozyme, the preparation method of which is as follows:

[0070] The graphyne oxide prepared in Example 1 was placed in a tube furnace and heated to 900° C. for 30 min under the protection of argon (flow rate of 200 sccm), and then fed into NH 3 Gas (flow rate: 20 sccm) was incubated at 900°C for 2h, and after cooling to room temperature with the furnace, nitrogen-doped graphyne nanozyme (N-GDY) was obtained.

[0071] The original graphyne nanozyme, oxidized graphyne nanozyme, and nitrogen-doped graphyne nanozyme are used as a method to simulate the catalytic activity of peroxidase:

[0072] (1) Disperse the original graphyne nanozyme, graphyne oxide nanozyme, and nitrogen-doped graphyne nanozyme into water respectively (the mass-volume ratio of graphyne nanozyme...

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Abstract

The invention discloses a nanozyme, a preparation method and its application, and belongs to the technical field of nanozyme catalysis, and solves the problem that the original graphyne is not used in the field of simulated enzyme catalysis in the prior art, and the catalytic activity of the simulated enzyme of the original graphyne is low. . The material of the nanozyme of the present invention contains graphyne, and the preparation method of the nanozyme comprises: step 1, preparing graphyne oxide; step 2, modifying graphyne oxide to obtain nitrogen-doped graphyne nanozyme or boron-nitrogen doped graphyne nanozyme. The nanozyme of the invention has high catalytic performance and wide application range.

Description

technical field [0001] The invention relates to the technical field of nanozyme catalysis, in particular to a nanozyme, a preparation method and an application thereof. Background technique [0002] Enzymes are a class of biocatalysts with high specificity and catalytic efficiency. Most natural enzymes are low in content, difficult to extract, high in cost, and prone to denaturation and loss of function when encountering non-physiological conditions such as heat, acid, and alkali. Nanozymes (nanozymes) are a class of artificial enzymes that not only have the catalytic function of enzymes, but also have the unique properties of nanomaterials. Nanozymes have the advantages of low preparation cost, high stability, easy storage, and large-scale preparation. They also have the advantages of designability and multifunctionality that natural enzymes do not have. They have broad application prospects in the fields of industry, agriculture, biomedicine, and the environment. . [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B32/21C01B32/23B01J21/18B01J27/24B82Y40/00
CPCB01J21/18B01J27/24B82Y40/00C01B32/21C01B32/23
Inventor 郭喜红黄换崔荣丽孙宝云
Owner 北京高能科迪科技有限公司
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