Lateral antibody chip for detecting diclofenac and indomethacin by using marker antibody probe

A technology of diclofenac and indomethacin, which is applied in the field of lateral antibody chips, can solve the problems of unstable quality, low sensitivity, weak detection signal intensity, etc., so as to improve detection sensitivity and quantitative precision, and improve detection signal strength and sensitivity. , the effect of increasing signal strength and stability

Inactive Publication Date: 2020-08-21
SHAOGUAN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has the following disadvantages: 1. To detect different target substances, it is necessary to use corresponding detection antibodies to connect quantum dot microspheres to prepare fluorescent probes, which requires a large workload, low yield, and unstable quality; The reaction to prepare fluorescent probes may affect the structure and immunoaffinity of antibodies; 3. Lack of signal amplification mechanism, the detection signal intensity is weak and the sensitivity is low
However, the immunogenicity of the "detection antigen carrier protein" is unstable, and slight changes in protein conformation will lead to a decrease in the affinity between the "universal antibody" and the "detection antigen", weaken the formation of "sandwich immune complexes", and affect the detection signal. produce

Method used

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  • Lateral antibody chip for detecting diclofenac and indomethacin by using marker antibody probe
  • Lateral antibody chip for detecting diclofenac and indomethacin by using marker antibody probe
  • Lateral antibody chip for detecting diclofenac and indomethacin by using marker antibody probe

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Experimental program
Comparison scheme
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Embodiment 1

[0039] A lateral antibody chip for detecting diclofenac and indomethacin with a marker antibody probe, comprising a backing plate 5, and a bifunctional antigen release pad 1, which is sequentially adhered to the backing plate 5 and partially overlapped between adjacent ones. Universal fluorescent probe release pad 2, nitrocellulose membrane 3 (chromatographic membrane), and absorbent pad 4; the dual-functional antigen release pad 1 contains detections that are coupled with ovalbumin, diclofenac and indomethacin, respectively On the basis of the antigen, the "7-amino-4-hydroxy-2-naphthalenesulfonic acid" label 62 is coupled; the universal fluorescent probe release pad 2 contains an anti-"7-amino-4-hydroxy-2-naphthalenesulfonic acid" label The universal fluorescent probe of “sulfonic acid” antibody 65, the universal fluorescent probe is quantum dot 61; the nitrocellulose membrane 3 is provided with a detection line A, a detection line B, and a quality control line 33, the detectio...

Embodiment 2

[0049] Simultaneous detection of diclofenac and indomethacin, the steps are as follows:

[0050] Take 0.2g (or 0.2mL) sample and dissolve it in 2.0mL absolute ethanol to fully extract the target test product. The extract is left for 10 minutes to fully precipitate impurities, and 0.2mL supernatant is added to 20mL sample buffer. Shake well to become a sample solution; use a dropper to add 3-4 drops of sample solution on the bifunctional antigen release pad. The sample solution will release the bifunctional antigen and the universal fluorescent probe during the movement of the sample solution to the nitrocellulose membrane, and then pass the detection. Line and quality control line, according to the fluorescence signal of detection line A, detection line B and quality control line to determine whether the sample contains diclofenac and indomethacin.

[0051] Refer to Figure 5 . The anti-diclofenac antibody 311 immobilized on the nitrocellulose membrane detection line A and the ant...

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Abstract

The invention relates to a lateral antibody chip for detecting diclofenac and indomethacin by using a marker antibody probe. The lateral antibody chip comprises a lining plate, and a difunctional antigen release pad, a universal fluorescent probe release pad, a chromatographic membrane and a water absorption pad which are sequentially adhered to the lining plate and are partially overlapped with one another, wherein the bifunctional antigen release pad comprises a specific marker coupled on the basis of a detection antigen formed by coupling ovalbumin with diclofenac and indomethacin respectively; the universal fluorescent probe release pad comprises a universal fluorescent probe labeled with an anti-specific marker antibody; the chromatographic membrane is provided with a detection line A, a detection line B and a quality control line which are respectively used for fixing an anti-diclofenac antibody, an anti-indomethacin antibody and the anti-specific marker antibody. According to the invention, the production of all detection reagents can be solved by using the universal fluorescent probe, the cost is reduced, and the quality control reliability is improved; and the signal intensity and the stability of detection are improved, and the detection sensitivity and the quantitative precision are improved.

Description

Technical field [0001] The invention relates to the field of food and drug safety detection, in particular to a lateral antibody chip for detecting diclofenac and indomethacin with a marker antibody probe. Background technique [0002] Both diclofenac and indomethacin are non-steroidal anti-inflammatory drugs with anti-inflammatory, analgesic and antipyretic effects. Because these two kinds of pain-relieving effects are rapid and cheap, illegal manufacturers illegally add diclofenac and indomethacin to health foods (mainly health wines) for rheumatic diseases to enhance the curative effect and gain illegitimate benefits. These two non-steroidal anti-inflammatory drugs are prescription drugs. Improper use may cause damage to the digestive tract, liver and kidneys, and even lead to anaphylactic shock and acute renal failure. Patients who take health foods containing diclofenac and indomethacin for a long time without knowing it are very harmful and must strengthen the supervision ...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/558
CPCG01N33/9486G01N33/582G01N33/588G01N33/558
Inventor郭杰标
OwnerSHAOGUAN COLLEGE