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Application of 5-lipoxygenase inhibitor and oncolytic virus in preparation of antitumor drugs

A technology of lipoxygenase and oncolytic virus, applied in the field of biomedicine, can solve the problems of different sensitivity of M1 virus and unsatisfactory oncolytic effect, and achieve the effect of good anti-tumor effect and minimization of toxicity.

Inactive Publication Date: 2020-09-01
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different tumors have different sensitivities to M1 virus, and for some tumors, when M1 virus is used alone, the oncolytic effect is not ideal.

Method used

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  • Application of 5-lipoxygenase inhibitor and oncolytic virus in preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 The combined treatment of Zileuton and M1 virus significantly reduces the survival rate of human hepatocellular carcinoma line Hep3B

[0032] Material:

[0033] Human hepatocellular carcinoma Hep3B (purchased from ATCC), M1 virus (accession number CCTCC V201423), high-glucose DMEM medium (purchased from Corning), multi-functional microplate reader.

[0034] method:

[0035] a) Inoculation of cells, administration treatment: select logarithmic growth phase cells, DMEM complete culture medium (containing 10% fetal bovine serum, 1% double antibody) to make cell suspension, inoculate with the density of 4 × 103 cells in each hole 96-well culture plate. After 12 hours, the cells were completely adhered to the wall. The experiment was divided into control group, Zileuton alone group, M1 infection group and Zileuton / M1 combination group. The doses used were: M1 virus (MOI=0.001) infected cells; Zileuton 12.5 μM.

[0036] b) Reaction between tetramethylazolium sal...

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Abstract

The invention discloses a 5-lipoxygenase inhibitor, which can be used as an anti-tumor synergist/drug resistance reversal agent of oncolytic virus for the first time. The invention provides an oncolytic virus synergist, which is low in toxicity and non-toxic to normal cells, and the oncolytic virus synergist can enable oncolytic viruses to play a better anti-tumor effect, and can minimize the toxicity of a pharmaceutical composition as much as possible while playing the effect of an anti-tumor drug.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and more specifically relates to the application of 5-lipoxygenase inhibitors and oncolytic viruses in the preparation of antitumor drugs. Background technique [0002] Oncolytic virus is a kind of replication-competent virus that targets and infects and kills tumor cells without damaging normal cells. Oncolytic virus therapy (oncolytic virotherapy) is an innovative tumor-targeted treatment strategy, which uses natural or genetically engineered viruses to selectively infect tumor cells and replicate in tumor cells to achieve targeted dissolution, The effect of killing tumor cells, but no damage to normal cells. [0003] Oncolytic virus M1 is a Getta-like virus of the Togaviridae alphavirus genus, isolated from mosquitoes of the genus Culex in Hainan Island, China. For example, Chinese invention patent application CN104814984A discloses that oncolytic virus M1 can selectively cause tumor cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K31/381A61K31/7088A61P35/00A61K35/761A61K35/763A61K35/768
CPCA61K31/381A61K31/7088A61K35/761A61K35/763A61K35/768A61K45/00A61P35/00
Inventor 蔡静林园梁剑开苏兴文
Owner SUN YAT SEN UNIV