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Treatment liquid for treating cerebral arterial thrombosis and preparation method and application thereof

A technology of ischemic stroke and treatment solution, which is applied in the field of treatment solution for ischemic stroke and its preparation, can solve the problems of limited treatment strategies for cerebral hypoxia, reduce infarct size, improve dysfunction, improve Effects on Cell Metabolism

Active Publication Date: 2020-09-08
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current therapeutic strategies to address cerebral hypoxia after stroke are limited

Method used

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  • Treatment liquid for treating cerebral arterial thrombosis and preparation method and application thereof
  • Treatment liquid for treating cerebral arterial thrombosis and preparation method and application thereof

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preparation example Construction

[0033] Such as figure 1 and figure 2 As shown, the present invention provides a kind of preparation method of ischemic stroke treatment liquid, comprises the following specific steps:

[0034] S1: culturing Synechococcus elongatus to obtain a resuspension of Synechococcus elongatus;

[0035] S2: modifying up-converting nanoparticles (UCNPs) to obtain a suspension of up-converting nanoparticles;

[0036] S3: Mix the resuspension of Synechococcus elongatus in the step S1 with the resuspension of upconversion nanoparticles in the step S2 in a volume ratio (1-10): 1 to obtain a treatment solution.

[0037] Further, the step S1 specifically includes the following steps:

[0038] S11: Set the concentration to (1-5)×10 6 Synechococcus elongatus culture solution and BG11 medium (blue-green medium) mixed in a volume ratio of 3:(4-6) per μL, followed by a cycle of 8 hours of light and 16 hours of darkness at 25-28°C Continuous training;

[0039] S12: Increase 3-4mL of BG11 medium...

Embodiment 1

[0065] The invention provides a method for preparing an ischemic stroke treatment liquid, comprising the following specific steps:

[0066] S1: set the concentration to 1×10 6 Individual / μL Synechococcus elongatus culture solution and BG11 medium are mixed according to the volume ratio of 3:4, and continuously cultured at 25°C for 8 hours of light and 16 hours of darkness cycle;

[0067] S2: Add 3 mL of BG11 medium every 4 days to offset the amount of evaporation, and continue culturing for 10 days;

[0068] S3: Discard 50% of the culture solution in step S2 when the concentration of the culture solution is supersaturated (i.e., precipitation or bacterial film occurs), and then increase the same volume of BG11 medium to continue culturing for 20 days to obtain a mixed culture solution;

[0069] S4: Take about 7mL of the mixed culture solution, centrifuge at 4000rpm for 10 minutes, discard the supernatant, and then add 200μL of phosphate buffered saline solution with a concent...

Embodiment 2

[0077] The invention provides a method for preparing an ischemic stroke treatment liquid, comprising the following specific steps:

[0078] S1: Set the concentration to 2×10 6 Synechococcus elongatus culture solution and BG11 culture medium of each / mL were mixed according to the volume ratio of 3:5, and were continuously cultured at 26°C for 8 hours of light and 16 hours of dark cycle;

[0079] S2: Add 3 mL of BG11 medium every 5 days to offset the amount of evaporation, and continue to culture for 11 days;

[0080] S3: Discard 50% of the culture solution in step S2 when the concentration of the culture solution is supersaturated (that is, precipitation or bacterial film occurs), and then increase the same volume of BG11 medium to continue culturing for 25 days to obtain a mixed culture solution;

[0081] S4: Take about 9 mL of the mixed culture solution, centrifuge at 5000 rpm for 12 minutes, discard the supernatant, and then add 300 μL of phosphate buffered saline solution ...

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Abstract

The invention relates to a treatment liquid for treating cerebral arterial thrombosis and a preparation method and application thereof. The treatment liquid is prepared by mixing an up-conversion nanoparticle resuspension solution and a synechococcus elongatus resuspension solution in a volume ratio of 1:(1-10). The invention further discloses a preparation method of the treatment liquid for cerebral arterial thrombosis. The method comprises the following specific steps: S1, culturing synechococcus elongatus to obtain a synechococcus elongatus resuspension solution; S2, carrying out modification treatment on up-conversion nanoparticles to obtain the up-conversion nanoparticle resuspension solution; and S3, mixing the synechococcus elongatus resuspension solution in the S1 with the up-conversion nanoparticle resuspension solution in the S2 in a proportion to obtain the treatment liquid. The treatment liquid has the beneficial effects that: the treatment liquid can effectively provide required oxygen for the brain of a human body, can improve the cell metabolic capability of hypoxic brain tissues to a certain extent, not only improves dysfunction caused by cerebral anoxia, but also can avoid injuries and risks caused by surgical craniotomy.

Description

technical field [0001] The invention relates to the field of tissue engineering and regenerative medicine, in particular to a treatment liquid for treating ischemic stroke and its preparation method and application. Background technique [0002] Stroke, commonly known as cerebral apoplexy, is an acute cerebrovascular disease. Stroke is currently the second leading cause of death and the leading cause of disability in the world. Globally, the number of deaths caused by stroke is about 6.5 million, and the number of new cases is about 10.3 million. Stroke with high morbidity, high mortality and high disability rate has caused great suffering to individuals and families, and also brought a serious burden to the country and society. Stroke is mainly divided into ischemic stroke and hemorrhagic stroke. Ischemic stroke is local brain tissue caused by the reduction or disappearance of cerebral artery blood supply caused by atherosclerosis, arteriolar disease or cardiac thrombosis...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P9/10C12N1/20C12R1/01
CPCA61K35/74A61P9/10C12N1/20
Inventor 王琳王征王健夏克尔扎提·肖哈拉提苏强飞
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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