Preparation process of probiotics for improving yin deficiency constitution and conditioning intestinal tracts

A preparation process and technology of probiotics, applied in the field of probiotics preparation, can solve the problems of low activity of lactic acid bacteria, low content of lactic acid bacteria, easy inactivation, etc., and achieve the effects of being beneficial to human body absorption, improving survival rate, and convenient operation

Inactive Publication Date: 2020-09-08
永奥(上海)健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are certain drawbacks in the existing lactic acid bacteria preparation process. The existing lactic acid bacteria preparation process usually directly uses dry powder lactic acid bacteria strains to add sugar and

Method used

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  • Preparation process of probiotics for improving yin deficiency constitution and conditioning intestinal tracts

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation process of probiotics for regulating intestinal tract for improving yin-deficiency constitution, comprising the following steps:

[0029] (1), dry powder lactic acid bacteria strains are placed on the culture medium, and then the culture medium is sent into the incubator for one-time cultivation to obtain lactic acid bacteria colonies;

[0030] MRS solid agar medium was used as the medium for primary culture, the culture temperature was 36° C., and the culture time was 42 hours.

[0031] (2), select the cultured lactic acid bacteria colonies, then send the lactic acid bacteria colonies into the incubator for secondary cultivation, obtain the lactic acid bacteria strains, and use the Gram staining method to screen out the bacterium colonies that meet the standard form of lactic acid bacteria;

[0032] During the secondary cultivation, the cultivation temperature of the incubator is 35°C, and the cultivation time is 17h;

[0033] (3) Inoculate the lactic ac...

Embodiment 2

[0044] A preparation process of probiotics for regulating intestinal tract for improving yin-deficiency constitution, comprising the following steps:

[0045] (4), dry powdered lactic acid bacteria strains are placed on the culture medium, and then the culture medium is sent into the incubator for one-time cultivation to obtain lactic acid bacteria colonies;

[0046] MRS solid agar medium was used as the medium for primary culture, the culture temperature was 36° C., and the culture time was 42 hours.

[0047] (5), select the cultured lactic acid bacteria colonies, then send the lactic acid bacteria colonies into the incubator for secondary cultivation, obtain the lactic acid bacteria strains, and use the Gram staining method to screen out the bacterium colonies that meet the standard form of lactic acid bacteria;

[0048] During the secondary cultivation, the cultivation temperature of the incubator is 35°C, and the cultivation time is 17h;

[0049] (6) Inoculate the lactic ...

Embodiment 3

[0060] A preparation process of probiotics for regulating intestinal tract for improving yin-deficiency constitution, comprising the following steps:

[0061] (7), dry powdered lactic acid bacteria strains are placed on the culture medium, and then the culture medium is sent into the incubator for one-time cultivation to obtain lactic acid bacteria colonies;

[0062] MRS solid agar medium was used as the medium for primary culture, the culture temperature was 36° C., and the culture time was 42 hours.

[0063] (8), select the cultured lactic acid bacteria colonies, then send the lactic acid bacteria colonies into the incubator for secondary cultivation, obtain the lactic acid bacteria strains, and use the Gram staining method to screen out the bacterium colonies that meet the standard form of lactic acid bacteria;

[0064] During the secondary cultivation, the cultivation temperature of the incubator is 35°C, and the cultivation time is 17h;

[0065] (9), inoculate the lactic...

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Abstract

The invention discloses a preparation process of probiotics for improving yin deficiency physique and conditioning intestinal tracts. The preparation process comprises the following steps: putting drypowder lactobacillus strains on a culture medium, and sending the culture medium into an incubator for primary culture to obtain lactobacillus colonies; and selecting cultured lactic acid bacteria colonies, and then feeding the lactic acid bacteria colonies into an incubator for secondary culture. Through the preparation process of probiotics for improving yin deficiency constitution and conditioning intestinal tracts, human gastric juice simulation can be performed on lactic acid bacteria, and then the lactobacillus strain adapts to the gastric juice environment of a human body, so that theprepared lactic acid bacteria can survive in the human body for a longer time, and absorption of the human body is facilitated. The lactic acid bacteria in fermentation are strains meeting the standard form of lactic acid bacteria, and fermentation culture is performed before fermentation, so that the lactic acid bacteria have gentle transition in the culture and fermentation processes, the survival rate of the lactic acid bacteria can be increased, the yield of the lactic acid bacteria can be increased, and a better use prospect is achieved.

Description

technical field [0001] The invention relates to the field of preparation of probiotics, in particular to a preparation process of probiotics for improving Yin-deficiency constitution and regulating intestinal tract. Background technique [0002] With the rapid development of society and the continuous improvement of people's living standards, people pay more and more attention to their own health. In order to improve people's physique, people have discovered some probiotics that can be used to improve Yin-deficiency physique and regulate the intestinal tract. There are lactic acid bacteria. In order to meet people's needs for lactic acid bacteria, people invented the preparation process of lactic acid bacteria; [0003] There are certain drawbacks in the existing lactic acid bacteria preparation process. The existing lactic acid bacteria preparation process usually directly uses dry powder lactic acid bacteria strains to add sugar and water for fermentation. It is easy to b...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/01
CPCC12N1/04C12N1/20
Inventor 刘小华
Owner 永奥(上海)健康科技有限公司
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