Use of selaginella tamariscina extract for improving gene expression, mitochondrial activity of cells, skin moisturizing ability and aging resistance

An extract, Selaginella technology, applied in the field of plant extracts, can solve problems such as harmfulness to human health, high price, etc., and achieve the effects of maintaining skin moisture, improving anti-aging, and enhancing mitochondrial activity.

Inactive Publication Date: 2020-09-22
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the known cosmetics, skin care products and health foods are made of chemical ingredients. Long-term use

Method used

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  • Use of selaginella tamariscina extract for improving gene expression, mitochondrial activity of cells, skin moisturizing ability and aging resistance
  • Use of selaginella tamariscina extract for improving gene expression, mitochondrial activity of cells, skin moisturizing ability and aging resistance
  • Use of selaginella tamariscina extract for improving gene expression, mitochondrial activity of cells, skin moisturizing ability and aging resistance

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0038] Example 1. Preparation of extract of Selaginella tamariscina

[0039] First, homogenize Selaginella tamariscina from Hangzhou Botai Plant Technology Co., Ltd., and then use water, alcohol, water-containing alcohol, or a combination thereof as the extraction solvent. The preferred extraction solvent is water. The homogenized Selaginella is subjected to a first stage extraction for 0.5 to 2 hours, wherein the temperature of the first stage extraction is between 50°C and 80°C, and the volume ratio of extraction solvent to Selaginella is between 10-20:1-5. And the extraction solvent has a hydrolase, and the hydrolase is a complex polysaccharase Viscozyme L (Novozyme Corp. produced by Novozymes). After the first stage of extraction, a crude extract is obtained. Next, perform a second stage extraction of the crude extract with the extraction solvent (preferably water) for 0.5 to 2 hours, wherein the temperature of the second stage extraction is between 50°C and 100°C, and the e...

Example Embodiment

[0040] Example 2. Evaluation of the effectiveness of Selaginella extract in enhancing the moisturizing ability of the skin

[0041] This example explores whether the Selaginella extract can achieve the effect of enhancing the moisturizing ability of the skin by regulating the gene expression related to the moisturizing of skin cells.

[0042] Culture human epidermal keratinocytes (HPEK-50; purchased from CELLnTEC) in a serum-free medium for keratinocytes (Keratinocyte-SFM; purchased from Thermo, product number: 17005042) in a 6-well plate, cell concentration of 2mL medium 1.5×10 5 Cells / wells.

[0043] After that, the cells were divided into 3 groups, including 1 control group and 2 experimental groups (ie, experimental groups 1 and 2). The extract of Selaginella was diluted with culture medium into diluents with concentrations of 0.03125mg / mL and 0.0625mg / mL, and then 0.03125mg / mL diluent was added to the cells of experimental group 1, and 0.0625mg / mL The diluent was added to the ...

Example Embodiment

[0051] Example 3. Evaluation of the effectiveness of Selaginella extract in enhancing the mitochondrial activity of cells

[0052] The present invention uses human skin fibroblasts to perform skin cell mitochondrial activity analysis. Human skin fibroblasts were purchased from the Bioresource Collection and Research Center (BCRC), Taiwan, China, under the number BCRC 60153. The cells were cultured in supplemented with 10% fetal bovine serum (FBS) (GIBCO, No. 10438-026, U.S.), 0.1 mM non-essential amino acids, 1.5 g / L sodium bicarbonate (Sigma, No. S5761 , U.S.), 1mM sodium pyruvate (GIBCO, No. 11360-070, U.S.) minimum essential medium (MEM) (Eagle) (prepared in Earle's Balanced Salt Solution, Earle 'S BSS)) (GIBCO Company, No. 41500-034, USA).

[0053] Inoculate 1×10 in each hole in a 6-well culture dish containing 2 mL of the above medium 5 Human skin fibroblasts (n=3). After that, the cells were divided into 3 groups, including 1 control group and 2 experimental groups (ie, ex...

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Abstract

The invention relates to the field of plant extracts, in particular to application of a selaginella tamariscina extract to improvement of gene expression quantity, mitochondrial activity of cells, moisturizing capacity of skin and ageing resistance. In the invention, the selaginella tamariscina extract is prepared by taking water, alcohols, hydrous alcohols or a combination thereof as an extraction solvent to carry out first-stage extraction on selaginella tamariscina to obtain a crude extract, and then carrying out second-stage extraction on the crude extract by using the extraction solvent,and the extraction solvent has a hydrolytic enzyme. The selaginella tamariscina extract can be used for preparing a composition for improving the gene expression quantity, a composition for improvingthe mitochondrial activity of cells and a composition for improving the skin moisturizing capacity and resisting aging.

Description

technical field [0001] The invention relates to the field of plant extracts, in particular to the use of an extract of Selaginella tamariscina for improving gene expression, mitochondrial activity of cells, improving skin moisturizing ability and anti-aging. Background technique [0002] The skin is the largest barrier to protect the human individual, and it has the function of resisting water loss, pathogenic bacteria and various environmental damages. Exposure to a large amount of ultraviolet rays (ultraviolet, UV), ionizing radiation, drugs or xenobiotics will promote the skin to generate reactive oxygen species (reactive oxygen species, ROS) and free radicals (free radicals). When the amount of accumulated reactive oxygen species and free radicals exceeds the antioxidant capacity of cells or tissues, oxidative stress will be formed. Then, reactive oxygen species and free radicals will react with intracellular components (including DNA, protein and lipids, etc.), and the...

Claims

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Application Information

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IPC IPC(8): A61K36/11A61P17/00A61K8/9741A61Q19/00A61Q19/08
CPCA61K36/11A61Q19/00A61Q19/08A61K8/9741A61P17/00
Inventor 林咏翔李姿仪
Owner TCI CO LTD
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