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Use of selaginella tamariscina extract for improving gene expression, mitochondrial activity of cells, skin moisturizing ability and aging resistance

An extract, Selaginella technology, applied in the field of plant extracts, can solve problems such as harmfulness to human health, high price, etc., and achieve the effects of maintaining skin moisture, improving anti-aging, and enhancing mitochondrial activity.

Inactive Publication Date: 2020-09-22
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the known cosmetics, skin care products and health foods are made of chemical ingredients. Long-term use is not only harmful to human health, but also these products are often expensive and cannot be afforded by ordinary users

Method used

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  • Use of selaginella tamariscina extract for improving gene expression, mitochondrial activity of cells, skin moisturizing ability and aging resistance
  • Use of selaginella tamariscina extract for improving gene expression, mitochondrial activity of cells, skin moisturizing ability and aging resistance
  • Use of selaginella tamariscina extract for improving gene expression, mitochondrial activity of cells, skin moisturizing ability and aging resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Preparation of the extract of Selaginella tamariscina

[0039] First, homogenize Selaginella tamariscina from Hangzhou Botai Plant Technology Co., Ltd., then use water, alcohols, hydroalcohols or a combination thereof as an extraction solvent. The preferred extraction solvent is water. The homogenized Selaginella is subjected to a first-stage extraction for 0.5-2 hours, wherein the temperature of the first-stage extraction is between 50°C and 80°C, and the volume ratio of the extraction solvent to Selaginella is 10-20:1-5, And the extraction solvent has a hydrolytic enzyme, and the hydrolytic enzyme is a complex polysaccharide enzyme Viscozyme L (produced by Novozyme Corp.). A crude extract was obtained after the first stage of extraction. Then, the crude extract is subjected to a second-stage extraction with the extraction solvent (preferably water) for 0.5 to 2 hours, wherein the temperature of the second-stage extraction is between 50°C and 100°C, and the...

Embodiment 2

[0040] Example 2. Efficacy evaluation of the extract of Selaginella in improving the moisturizing ability of the skin

[0041] This example is to explore whether the extract of Selaginella can improve the skin's moisturizing ability by regulating the expression of genes related to skin cell moisturizing.

[0042] Human epidermal keratinocytes (HPEK-50; purchased from CELLnTEC) were cultured in keratinocyte-specific serum-free medium (Keratinocyte-SFM; purchased from Thermo, product number: 17005042) in a 6-well plate, the cell concentration of 2 mL medium 1.5×10 5 cells / well.

[0043] Afterwards, the cells were divided into 3 groups, including 1 control group and 2 experimental groups (ie, experimental groups 1 and 2). The extract of Selaginella chinensis was diluted with medium into dilutions having concentrations of 0.03125 mg / mL and 0.0625 mg / mL, and then 0.03125 mg / mL dilutions were added to the cells of experimental group 1, and 0.0625 mg / mL The dilutions were added to...

Embodiment 3

[0051] Example 3. Evaluation of the effectiveness of the extract of Selaginella in enhancing the mitochondrial activity of cells

[0052] The invention uses human skin fibroblasts to analyze the mitochondrial activity of skin cells. Human dermal fibroblasts were purchased from Taiwan Bioresource Collection and Research Center (BCRC), number BCRC 60153. The cells were cultured in a medium supplemented with 10% fetal bovine serum (fetal bovine serum, FBS) (GIBCO company, No. 10438-026, U.S.), 0.1 mM non-essential amino acids, 1.5 g / L sodium bicarbonate (Sigma company, No. S5761 , the United States), 1mM sodium pyruvate (GIBCO company, No. 11360-070, the United States) minimum essential medium (Minimum essential medium, MEM) (Eagle) (mixed with Earle's Balanced Salt Solution (Earle's Balanced Salt Solution, Earle 's BSS)) (GIBCO Corporation, No. 41500-034, USA).

[0053] Inoculate 1×10 per well in a 6-well culture plate containing 2 mL of the above medium 5 Individual human de...

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Abstract

The invention relates to the field of plant extracts, in particular to application of a selaginella tamariscina extract to improvement of gene expression quantity, mitochondrial activity of cells, moisturizing capacity of skin and ageing resistance. In the invention, the selaginella tamariscina extract is prepared by taking water, alcohols, hydrous alcohols or a combination thereof as an extraction solvent to carry out first-stage extraction on selaginella tamariscina to obtain a crude extract, and then carrying out second-stage extraction on the crude extract by using the extraction solvent,and the extraction solvent has a hydrolytic enzyme. The selaginella tamariscina extract can be used for preparing a composition for improving the gene expression quantity, a composition for improvingthe mitochondrial activity of cells and a composition for improving the skin moisturizing capacity and resisting aging.

Description

technical field [0001] The invention relates to the field of plant extracts, in particular to the use of an extract of Selaginella tamariscina for improving gene expression, mitochondrial activity of cells, improving skin moisturizing ability and anti-aging. Background technique [0002] The skin is the largest barrier to protect the human individual, and it has the function of resisting water loss, pathogenic bacteria and various environmental damages. Exposure to a large amount of ultraviolet rays (ultraviolet, UV), ionizing radiation, drugs or xenobiotics will promote the skin to generate reactive oxygen species (reactive oxygen species, ROS) and free radicals (free radicals). When the amount of accumulated reactive oxygen species and free radicals exceeds the antioxidant capacity of cells or tissues, oxidative stress will be formed. Then, reactive oxygen species and free radicals will react with intracellular components (including DNA, protein and lipids, etc.), and the...

Claims

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Application Information

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IPC IPC(8): A61K36/11A61P17/00A61K8/9741A61Q19/00A61Q19/08
CPCA61K36/11A61Q19/00A61Q19/08A61K8/9741A61P17/00
Inventor 林咏翔李姿仪
Owner TCI CO LTD
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