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Method for determining contents of paeoniflorin, cinnamaldehyde and paeonol in traditional Chinese medicine preparation

A technology of traditional Chinese medicine preparations and determination methods, which is applied to the content determination of paeonol, cinnamon aldehyde, and paeoniflorin in traditional Chinese medicine preparations, can solve the problems that Guizhi muscle tumor pills cannot be inspected and overall quality control, and achieve time saving and The effect of detection cost, reduction of operation steps and consumption of reagents, improvement of accuracy

Active Publication Date: 2020-09-25
曹薇薇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention proposes a content determination method of paeoniflorin, cinnamon aldehyde and paeonol in traditional Chinese medicine preparations, which solves the problem that the quality inspection and overall quality control of Guizhi muscle tumor pills in the prior art cannot be carried out

Method used

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  • Method for determining contents of paeoniflorin, cinnamaldehyde and paeonol in traditional Chinese medicine preparation
  • Method for determining contents of paeoniflorin, cinnamaldehyde and paeonol in traditional Chinese medicine preparation
  • Method for determining contents of paeoniflorin, cinnamaldehyde and paeonol in traditional Chinese medicine preparation

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Experimental program
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Embodiment 1

[0062] A. After chopping the sample of Guizhi muscle tumor pills, accurately weigh 0.6024g of the sample and place it in a stoppered conical flask, add 25ml of mixed solvent A to dissolve to obtain solution Ⅰ, seal the conical flask tightly, weigh it, and record for m 1 , Ultrasonic treatment of solution I, ultrasonic treatment power 240w, frequency 45kHz, time 20min, after ultrasonic treatment, cooled to room temperature to obtain solution II, weighed again, recorded as m 2 , using mixed solvent A to reduce the weight of solution II by m 2 make up to m 1 , and then filtered through a 0.45 μm microporous membrane to obtain a sample solution;

[0063] Wherein the component of the mixed solvent A is composed of 5% triethylene glycol and 95% methanol by mass percentage;

[0064] B. Precisely weigh 18.76mg of paeoniflorin reference substance, 18.63mg of cinnamon aldehyde reference substance, and 10.13mg of paeonol reference substance, place them in 50ml, 100ml, and 50ml volumet...

Embodiment 2

[0076] A. After chopping the sample of Guizhi muscle tumor pills, accurately weigh 0.8064g of the sample and place it in a stoppered conical flask, add 25ml of mixed solvent A to dissolve to obtain solution Ⅰ, seal the conical flask tightly, weigh it, and record for m 1 , Ultrasonic treatment of solution I, ultrasonic treatment power 270w, frequency 55kHz, time 40min, after ultrasonic treatment, cooled to room temperature to obtain solution II, weighed again, recorded as m 2 , using mixed solvent A to reduce the weight of solution II by m 2 make up to m 1 , and then filtered through a 0.45 μm microporous membrane to obtain a sample solution;

[0077] Wherein the mixed solvent A component is composed of 15% triethylene glycol and 85% methanol by mass percentage;

[0078] B, make mixing reference substance solution A, B, C, D, E, F by the preparation method among the embodiment 1;

[0079] C, the sample solution obtained in step A and the mixed reference substance solution A, ...

Embodiment 3

[0085] A. After chopping the sample of Guizhi muscle tumor pills, accurately weigh 0.8992g of the sample and place it in a stoppered conical flask, add 25ml of mixed solvent A to dissolve to obtain solution Ⅰ, seal the conical flask tightly, weigh it, and record for m 1 , Ultrasonic treatment of solution I, ultrasonic treatment power 250w, frequency 50kHz, time 30min, after ultrasonic treatment, cooled to room temperature to obtain solution II, weighed again, recorded as m 2 , using mixed solvent A to reduce the weight of solution II by m 2 make up to m 1 , and then filtered through a 0.45 μm microporous membrane to obtain a sample solution;

[0086] Wherein the component of the mixed solvent A is composed of 10% triethylene glycol and 90% methanol by mass percentage;

[0087] B, make mixing reference substance solution A, B, C, D, E, F by the preparation method among the embodiment 1;

[0088] C, the sample solution obtained in step A and the mixed reference solution A, B...

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Abstract

The invention relates to the technical field of medicine determination, and provides a method for determining the content of paeoniflorin, cinnamaldehyde and paeonol in a traditional Chinese medicinepreparation. The method comprises the following steps: weighing a sample, dissolving by using a mixed solvent A, carrying out ultrasonic treatment, cooling, fixing the volume by using the mixed solvent A, and filtering to obtain a sample solution, wherein the mixed solvent A is prepared from the following components in percentage by mass: 5-15% of triethylene glycol and 85-95% of methanol; weighing paeoniflorin, cinnamaldehyde and paeonol reference substances, dissolving by using the mixed solvent A, fixing the volume, respectively taking as reference substance stock solutions a, b and c, anddiluting by using the mixed solvent A to obtain mixed reference substance solutions with gradient concentration; carrying out high performance liquid chromatography analysis on the sample solution, and recording a chromatogram; and calculating the contents of paeoniflorin, cinnamyl aldehyde and paeonol in the sample. By means of the technical scheme, the problems that in the prior art, quality detection and overall quality control cannot be conducted on cassia twig myoma pills are solved.

Description

technical field [0001] The invention relates to the technical field of drug determination, and provides a content determination method for paeoniflorin, cinnamic aldehyde and paeonol in traditional Chinese medicine preparations. Background technique [0002] Guizhi muscle tumor pill (Z20050873, Jiyao Zhizi) is a preparation of Yutian County Hospital of Traditional Chinese Medicine, Hebei Province. Honeyed pills have the functions of promoting blood circulation, removing blood stasis, and dissipating blockages. Used for blood stasis caused by uterine leakage, purplish dark gray, abdominal pain and refusal to press; uterine fibroids, dysmenorrhea, pelvic inflammatory disease and other diseases. The six medicinal materials have many chemical components and play different pharmacological effects. Among them, paeoniflorin and paeonol are the main components shared by Radix Paeoniae Rubra and Moutan Cortex, and cinnamic aldehyde is the main component of cassia twig. Paeoniflorin...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34G01N30/74G01N30/86
CPCG01N30/02G01N30/34G01N30/8631G01N30/74
Inventor 曹薇薇
Owner 曹薇薇
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