Hepatitis B surface antigen detection kit

A technology for detecting kits and surface antigens, applied in the field of immunoanalysis medicine, which can solve the problems of poor accuracy and precision of detection results, high-efficiency pollution of DNA amplification, cumbersome operation process, etc., and achieve the effect of low detection cost

Pending Publication Date: 2020-09-25
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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  • Claims
  • Application Information

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Problems solved by technology

[0004] Enzyme-linked immunosorbent assay (ELISA) is widely used because of its simple operation, no radioactive pollution, and low price, but its sensitivity is low
The detection process is mostly manual operation, the addition of reagents or samples is not very precise, the oper

Method used

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Examples

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preparation example Construction

[0093] The preparation method of magnetic particle-HBsAg antibody (preparation 1L) is as follows,

[0094] Take 100mL of 0.1M hydroxyethylpiperazine ethylsulfuric acid (HEPES) buffer solution, add 80mg of magnetic particles with amino or carboxyl groups on the surface, stir at room temperature for 40min, then add 10mg of HBsAg antibody, and then add EDC at a concentration of 8mg / mL, After reacting at 2-8°C for 1 hour, wash with 0.01M PBS buffer for 3 times, and finally dilute to 1L with 0.01M PBS.

[0095] Analysis process:

[0096] 1. Take out an appropriate amount of reaction tubes according to the needs of the experiment. Set up 2 tubes of each calibrator and 2 tubes of each quality control product.

[0097] 2. Add 75 μL of reagent 1 prepared by alkaline phosphatase-labeled F(ab')2HBsAg goat polyclonal antibody to each tube.

[0098] 3. Add 75 μL of calibrator, quality control or sample to each tube.

[0099] 4. React at 37°C for 20 minutes.

[0100] 5. Add 50 μL magne...

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Abstract

The invention provides a hepatitis B surface antigen detection kit. The kit comprises an alkaline phosphatase labeled F (ab') 2HBsAg sheep polyclonal antibody and a magnetic particle-HBsAg antibody. In the kit disclosed by the invention, the antibody directly coats the magnetic particles, so the reaction efficiency is better, and the sensitivity is favorably improved; some non-specific binding occurs at the FC end of the antibody, and the molecular weight of the cut antibody Fab2 is smaller, so the sensitivity is improved.

Description

technical field [0001] The invention belongs to the technical field of immune analysis medicine, in particular to a detection kit for hepatitis B surface antigen. Background technique [0002] Hepatitis B Virus Surface Antigen (HBsAg) is the coat protein of hepatitis B virus. It consists of 226 amino acid residues, with a relative molecular mass of 24,000 (glycosylated form of 27,000). In the serum of HBV-infected patients, it exists in the form of small round particles with a diameter of 17-25 nm and tubular or filamentous particles of varying lengths. [0003] HBsAg exists not only in blood, but also in many body fluids and secretions, such as saliva, milk, sweat, tears, urine, nasopharyngeal secretions, semen, etc. Serum HBsAg is only a sign of HBV infection, and cannot reflect whether the virus has replicated, the degree of replication, the strength of infectivity and prognosis. Most patients with acute hepatitis B can turn negative early in the course of the disease,...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/543G01N33/535
CPCG01N33/5764G01N33/54306G01N33/535G01N33/54326
Inventor 张振斌李华史光辉栾大伟刘萍
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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