Stem cell extract and preparation method and application thereof

A stem cell and extract technology, applied in the field of stem cell extract and its preparation, to achieve the effect of improving inflammation and ingredient safety

Pending Publication Date: 2020-10-13
博品(上海)生物医药科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Human umbilical cord mesenchymal stem cells (hucMSCs) are considered a promising therap

Method used

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  • Stem cell extract and preparation method and application thereof
  • Stem cell extract and preparation method and application thereof
  • Stem cell extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Embodiment two

[0024] On the basis of Example 1, when inducing culture, 25 mL of physiological saline containing 0.2 μL vitamin E was used, no other substances were added, and the rest remained unchanged. Parallel experiments were carried out. figure 1 , figure 2 2 of them.

Example Embodiment

[0032] Embodiment Three

[0033] Through animal experiments, the stem cell extract stock solution of the present invention can alleviate the symptoms of pulmonary fibrosis, and the mice are from Harbin Veterinary Research Institute.

[0034]A single high-dose intratracheal bleomycin injection was used to create a mouse model of pulmonary fibrosis (6 weeks old, modeled by conventional methods, and it was checked to confirm that the model was successful). Ratio 2 (control group) was compared, and a normal saline group (blank group) was set at the same time; the improvement of pulmonary fibrosis symptoms was investigated, with 10 model mice in each group.

[0035] On the 10th day after the model was established, nebulization treatment was started. The stem cell extract of Example 1, the solution of Comparative Example 2 and normal saline were used respectively, and 3 mL of liquid was added to the nebulizer each time, and an air-compressed nebulizer was used. The atomization time...

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Abstract

The invention discloses a stem cell extract and a preparation method and application thereof. The preparation method includes the following steps that primary stem cells are inoculated in a culture medium, primary culturing is carried out, a solution is changed every other day, when the primary stem cells grow to 80-90%, the medium is removed, the remaining stem cells are rinsed and then mixed with physiological saline, and after the induced culture is carried out, centrifugal separation is carried out to obtain a supernatant; and then the supernatant is filtered to obtain the stem cell extract. According to the stem cell extract and the preparation method and application thereof, more safety is achieved due to non-exogenous animal components, and the obtained extract presents abundant cytokines and can be directly used as a pharmaceutical preparation or mixed with adjuvants after freezing to prepare a medicine.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a stem cell extract and a preparation method and application thereof. Background technique [0002] In the actual working scene of cells, most molecules (such as proteins) in cells are too large to directly pass through the membrane structure in cells. Therefore, energy conversion, information recognition and transmission between cells need to be performed all the time. Basic life processes such as material transportation. In recent years, with the rapid development of science and technology, as one of the vesicles, exosomes participate in the important regulation of cell activities, and have emerged in the treatment of various intractable diseases. Exosomes are a kind of lipid bilayer membranous vesicles produced by various living cells through a series of biological mechanisms such as endocytosis-fusion-efflux, and expelled from the cell membrane by active sec...

Claims

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Application Information

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IPC IPC(8): A61K35/51A61P29/00C12N5/0775
CPCA61K35/51A61P29/00C12N5/0668
Inventor 董健伸徐汝强孙永沛程洪斌王晓东徐俊
Owner 博品(上海)生物医药科技有限公司
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