High-survival-rate sheep fertilized egg culture method for Hu sheep embryo in-vitro culture

Abstract

The invention discloses a high-survival-rate sheep oosperm culture method for Hu sheep embryo in-vitro culture. The high-survival-rate sheep oosperm culture method comprises the following steps: (1) oocyte collection: sucking oocytes from ovaries of Hu sheep; (2) sperm collection: extracting sperms from the male Hu sheep; (3) fertilization: carrying out artificial fertilization on eggs and sperms;(4) embryo culture: putting the fertilized eggs into an incubator for culture; and (5) observation: observing the development condition of the embryo, wherein the incubator in the step (4) comprisesa main body, a feeding device, a rotating frame, a culture device and a discharging device, wherein the feeding device comprises a feeding channel, a conveying mechanism, a feeding opening, a rotatingtable and a transition mechanism. According to the method, the embryo survival rate is increased through artificial fertilization, the transition mechanism isolates the embryos from outside air whenthe embryos are placed into the incubator, so that embryo development is prevented from being influenced, and the discharging device realizes the purpose of independent taking a culture dish, so thatthe survival rate of embryos is increased.

Description

technical field [0001] The invention belongs to the technical field of in vitro culture of sheep embryos, in particular to a method for culturing fertilized sheep eggs with high survival rate for in vitro culture of Hu sheep embryos. Background technique [0002] With the development of modern reproductive biotechnology and the gradual improvement of in vitro embryo production technology, the in vitro production technology of sheep embryos has become an important way to speed up the breeding of breeds and the expansion of fine populations; although live offspring have been obtained through in vitro embryo production technology , but in vitro embryos obtained by in vitro fertilization generally have developmental blockage during the culture process, resulting in a much lower blastocyst development rate of in vitro embryos than in vivo embryos; the current nutritional environment of Hu sheep embryos cultured in vitro is the key reason for the above problems One, for example, i...

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Problems solved by technology

[0002] With the development of modern reproductive biotechnology and the gradual improvement of in vitro embryo production technology, the in vitro production technology of sheep embryos has become an important way to speed up the breeding of breeds and the expansion of fine populations; although live offspring have been obtained through in vitro embryo production technology , but in vitro embryos obtained by in vitro fertilization generally have developmental blockage during the culture process, resulting in a much lower blastocyst development rate of in vitro embryos than in vivo embryos; the current nutritional environment of Hu sheep embryos cultured in vitro is the key reason for the above problems One, for example, in the process of checking the fertilized eggs of sheep, the fertilized eggs need to be taken out of the incubator, which has a great impact on the temperature, humidity, oxygen, carbon dioxide, etc. in the incubator

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