Method, device, computer equipment and storage medium for predicting protein expression

Abstract

The present application relates to a method, device, computer equipment and storage medium for predicting protein expression. Including: obtaining the grayscale images of the cells to be tested respectively corresponding to a plurality of cells to be tested in the cell culture pool; inputting the grayscale images of the cells to be tested into the target generation network model; the target generation network model adopts multiple training cell grayscale The graph is obtained by training the generative confrontation network model; the multiple training cell grayscale images have corresponding fluorescent image labels; the fluorescent image labels are the real fluorescent images corresponding to the training cell grayscale images; the network is generated according to the target The output of the model is to obtain the predicted fluorescence maps corresponding to the plurality of cells to be tested; according to the predicted fluorescence maps, the protein expression levels corresponding to the plurality of cells to be tested are determined, and the rapid determination of The protein expression of each cell in the culture pool avoids repeated culture and screening, and effectively improves the prediction efficiency of the protein expression of cells during the culture process.

Description

technical field [0001] The present application relates to the field of biotechnology, in particular to a method, device, computer equipment and storage medium for predicting protein expression. Background technique [0002] With the continuous development of genetic engineering technology, it has become a common requirement in the biological field to isolate monoclonal cell lines capable of expressing specific products from cell pools. [0003] In the prior art, the cells in the cell pool can be transfected, and the cell pool can be treated by the limiting dilution method to obtain single cells, and then the single cells can be used to culture a homogeneous cell population, that is, a cell line, And test the expression level of the target protein in the cell line, and screen the cell line with high expression level of the target protein. [0004] However, the limited dilution method is cumbersome and requires repeated cell culture and screening before calculating the expres...

AI Technical Summary

Problems solved by technology

[0004] However, the limited dilution method is cumbersome and requires repeated cell culture and screening before calculating the expression of the target protein, resulting in low efficiency and time-consuming prediction of protein expression.

Images