Reagent for overcoming self-incompatibility of Brassica and Radish and its usage and application
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A technology of the genus Radish and Brassica, applied in the field of breeding, can solve problems such as unfavorable promotion
Active Publication Date: 2021-04-20
SHANDONG AGRICULTURAL UNIVERSITY
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Embodiment 1
[0024]Example 1 as a case of Chinese cabbage
[0025]Big cabbage picks up the open flowers before spraying salicylate, and the bag is set. On the second morning, the sodium salicylate solution was sprayed (sodium salicylate solution containing 0.025% of Tween 20, sodium salicylate was 0 mm, 1 mm, 3 mm, 5 mm), each spray 5 Under -7, co-spray 2 times, separated by 30 min, and after the second spraying is dry after the pillar is dried, and the collected belt prevents pollution other pollen. Fruit pod anatomy, statistical and index is performed after 20-30 days, see Table 1.
[0026]Table 1. Affinity index of Chinese cabbage after spraying salicylate
[0027]
[0028]byFigure 1 - Figure 6 , And Table 1, we can draw: Self-flowering led to the rapid oxygen rose, corresponding to self-intersession, and abuse pollination leads to a decrease in the active oxygen of the Chinese cabbage column, corresponding to the foreign affinity (figure 1 ), Indicating that the column active oxygen has an inhibitory ef...
Embodiment 2
[0029]Example 2 as an example of radish
[0030]The radish removing the already open flowers before spraying salicylate, and the bag is collected. The next morning, the sodium sodium salicylate solution was sprayed (sodium salicylate solution containing 0.025% of Tween 20, sodium salicylate concentration was 0 mm, 2 mm, 5 mm, 10 mm), each spray 5 Under -7, co-spray 2 times, separated by 30 min, and after the second spraying is dry after the pillar is dried, and the collected belt prevents pollution other pollen. 20-30 days later, pod anatomy, statistical proxires, see Table 2
[0031]Table 2. Affinity of the radish after spraying salicylate
[0032]
[0033]byFigure 7 - Figure 11And Table 2, we can see that it is similar to Chinese cabbage. Self-prone isas, leading to the active oxygen rises of radish column, corresponding to self-traveled, and is a decrease in the active oxygen of radish column, corresponding to DIS (Figure 7 ), Indicating that the column active oxygen has an inhibitory effect...
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Abstract
The invention relates to a reagent for overcoming the self-incompatibility of Brassica and Radish, as well as its usage and application. The present invention relates to the technical field of breeding of plants of the genus Brassica and radish. Taking Chinese cabbage and radish as an example, when the Chinese cabbage and radish enter the initial flowering stage and bloom in large quantities, during the time period of 8:00-10:00 in the morning on a sunny day, Spray the 1 mM-10mM sodium salicylate solution mixed with 0.025% Tween-20 (increasing the adhesion of the solution) on the stigma that has just unfolded into a cross flower with a sprayer, with an interval of 30 minutes in between, and spray for the second time , Spray 5‑7 times each time, and mist droplets can be seen on the stigma. After the second spraying, the mist droplets on the stigma are all dry, and then fresh pollen is applied. The method of the present invention is applicable to the self-incompatibility line of Brassica plants and the self-incompatibility line of Radish plants. to Chinese cabbage ( Brassica rapa L. ssp. pekinensis ) and radish ( Raphanus sativus ) of the self-incompatibility line was sprayed with sodium salicylate solution on the stigma of the cruciform stigma, which could improve the self-compatibility index to varying degrees.
Description
Technical field[0001]The present invention belongs to the field of breeding, and more particularly to a reagent and usage and application of overcoming plant self-employment.Background technique[0002]Self-intensity is a genetic mechanism that prevents auto-pollination from promoting the pollination of abundance to promote the adaptability of autonomic recession in the prevention of autonomous recession. According to the source of male identification factors, self-trade non-affinity is divided into spores, and the body is self-tradition. So far, the spore bodies represented by the 薹 不 自 亲 有 研究 研究 研究 在 中 中 中 中 中 在 在 在 在 在 在 在 在 在 在 在 中-在 中 因 因 在 中 在 中 中 中 中The cell type pollen signal molecule SP11 / SCR is combined to excite the umoid cell to produce a rejection reaction, inhibit the flour. Radish vegetable crops such as Chinese cabbage and radish are typical spore bodies. They do not affinity plants. It is designed to be sprouting from pollen, or germination, or germination, it is wr...
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