Application of interfering expression of lcyb and lcye while overexpressing pap in preparing Brassica plants with red petals

A technology of overexpression and Brassica, applied to the field of application in the preparation of Brassica plants with red petals

Inactive Publication Date: 2017-05-31
SOUTHWEST UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the entire Brassica genus including rapeseed, there is no report on the use of molecular breeding for metabolic engineering of rapeseed flower color.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of interfering expression of lcyb and lcye while overexpressing pap in preparing Brassica plants with red petals
  • Application of interfering expression of lcyb and lcye while overexpressing pap in preparing Brassica plants with red petals
  • Application of interfering expression of lcyb and lcye while overexpressing pap in preparing Brassica plants with red petals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Microscopic observation of chromogenic subcellular organelles in Brassica and radish petals

[0042] Take fresh Brassica napus yellow petals (BnY); Brassica napus milk petals (BnW); Brassica napus yellow petals (BjY); Brassica napus yellow petals (BrY); White petals (BoW) and radish red petals (RsR) were stored on ice and shipped back to the laboratory, then sliced ​​by hand, observed with a low-power lens for quick screening, and qualified slices were selected for careful observation and photography. The results are as follows figure 1 shown. The results showed that the yellow substances in the yellow petals of several species of Brassica appeared as numerous small granules in one cell, distributed unevenly in the cell, and were squeezed to the wall-adhering position by the vacuoles, indicating that the yellow petals The chromogenic organelles are chromoplasts, whose pigments are yellow carotenoids. There are also some cells in the milky petals of Brassica...

Embodiment 2

[0043] Embodiment 2, detect brassica and radish petal pigment

[0044] In the morning of the full flowering period, the newly opened Brassica napus yellow petals (BnY); Brassica napus milk petals (BnW); Brassica napus yellow petals (BjY); BoY), kale white petals (BoW) and radish red petals (RsR), immediately transported back to the laboratory in a cool and fresh place, dried at 50-60°C, ground into powder, passed through a 60-mesh sieve, and stored in a dry place away from light.

[0045] 1. Test results of petroleum ether, hydrochloric acid and ammonia water

[0046] Weigh 0.100g of preserved petal powder, put them into numbered stoppered test tubes, add about 10mL of petroleum ether, 10% hydrochloric acid, and 30% ammonia water, mix gently, filter, and observe the color change. The results are as follows :

[0047] (1) Petroleum ether reaction: Brassica napus, Chinese cabbage rape, and mustard rape all show bright yellow, indicating that the content of carotenoids is high;...

Embodiment 3

[0067] Example 3. Molecular Identification of Brassica and Radish Petal Pigment Biosynthetic Pathways

[0068] (1) Expression characteristics of flavonoid pathway genes in Brassica and radish petals

[0069] In order to study the molecular mechanism of the difference in petal pigments between Brassica and radish, RT-PCR detection primers for the flavonoid pathway genes of Brassica and radish were designed, and 5SrRNA was used as an internal reference, as shown in Table 1:

[0070] Table 1. Primers for RT-PCR detection of flavonoid pathway

[0071]

[0072]

[0073]In the morning of the full flowering period, the newly opened Brassica napus yellow petals (BnY); Brassica napus milk petals (BnW); Brassica napus yellow petals (BjY); BoY), kale with white petals (BoW) and red radish petals (RsR), transported frozen in liquid nitrogen, and stored in a -80°C refrigerator for later use. Then use the RNAprep Pure plant total RNA extraction kit to extract the total RNA, after el...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses application of a gene for interfering expression of LCYB and LCYE genes and simultaneously over-expressing a PAP gene in preparation of a brassica plant having red petals. The invention further discloses an expression vector for interfering expression of the LCYB and LCYE genes and simultaneously over-expressing the PAP gene and a preparation method of the expression vector. Yellow carotenoid is inhibited and red lycopene is accumulated by interfering expression of the LCYB and LCYE genes; accumulation of cyanin can be promoted through over-expression of the PAP gene; therefore, the petals are red; the brassica plant having red petals is successfully prepared; and colours of the brassica plants are enriched.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of interfering with the expression of LCYB and LCYE genes while overexpressing the PAP gene in preparing brassica plants with red petals. Background technique [0002] Rapeseed is an important oil crop with a long history of cultivation, high economic value, wide application and strong adaptability. It is the largest oil crop in my country and an important oil crop worldwide. In terms of species taxonomy, rape is composed of three species of Brassica, Brassica napus (2n=38, AACC), Brassica rapassp.oleifera syn.B. campestris, 2n=20, AA), Brassica juncea (2n=36, AABB). Brassica napus is a compound species evolved from natural interspecific hybridization of Chinese cabbage (Brassica rapa) and cabbage (Brassica oleracea, 2n=18, CC), although the history of cultivation is only a few hundred years , but because of its strong growth potential and high yield, it h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/66C12N15/84A01H5/00A01H4/00
Inventor 柴友荣金筱耘林呐付春尹能文练剑平蒋佳怡廖霏霏刘雪孔志强
Owner SOUTHWEST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap