Brassica napus TT10 gene families and application thereof

A technology of Brassica napus and gene family, which is applied in the field of genetic engineering and achieves the effect of good application prospects

Inactive Publication Date: 2012-07-25
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the number of members, protein characteristics, evolutionary relationship, tissue specificity of expression, and relationship with yellow seed traits of TT10 gene in Brassica napus and its parent species, Chinese cabbage and Brassica oleracea.

Method used

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  • Brassica napus TT10 gene families and application thereof
  • Brassica napus TT10 gene families and application thereof
  • Brassica napus TT10 gene families and application thereof

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Embodiment Construction

[0033] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0034] The plant material used in the preferred embodiment: the Chinese cabbage material all comes from the Brassica napus subspecies (B.rapa ssp.oleifera), including the typical black seed line 06K130 and the yellow and black seed near-isogenic lines 09L597 (black seed) and 09L600 ( oleracea var.acephala), including the typical black-seed line 06K158 and the near-isogenic lines 09gan 1 (black seed) and 09gan 4 (yellow seed ); Brassica napus materials include typical black seed maintainer line 5B...

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Abstract

The invention discloses brassica napus and parent species thereof Chinese cabbage and brassica oleracea TT10 gene families and application thereof. The Chinese cabbage TT10 gene family comprises a BrTT10-1A gene (SEQ ID No.1-2), a BrTT10-1B gene (SEQ ID No.3-4) and a BrTT10-2 gene (SEQ ID No.5-6); the brassica oleracea TT10 gene family comprises a BoTT10-1 gene (SEQ ID No.7-8) and a BoTT10-1pse pseudo-gene (SEQ ID No.9); and the brassica napus gene family comprises a BnTT10-1 gene (SEQ ID No.10-11), a BnTT10-2 gene (SEQ ID No.12) and a BnTT10-3 gene (SEQ ID No.13-14). The gene families can beapplied to molecular breeding of brassica crop seed characters.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to Brassica napus and its parent species Chinese cabbage (Brassica rapa) and cabbage (Brassica oleracea) TT10 (TRANSPARENT TESTA10, transparent seed coat 10; also known as LAC15, namely LACCASE 15 , laccase 15) gene family and applications thereof. Background technique [0002] The genus Brassica (Brassica) of the Brassicaceae (Brassicaceae) includes many oil crops, vegetables and ornamental plant species. It provides humans with nutritious edible oils, vegetables and ornamental plants, and provides feed for animal husbandry. It has an important economic value. Among the Brassica species, the allotetraploid species Brassica napus was formed from two diploid species Brassica oleracea and Brassica oleracea through interspecific hybridization followed by remultiplication. Brassica napus is the second largest oil crop in the world, widely planted all over the world, and i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C12N5/10A01H5/00
Inventor 柴友荣李加纳梁颖张凯卢坤曲存民王瑞王敬乔陈薇
Owner SOUTHWEST UNIV
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