Application of Elabela polypeptide in preparation of antioxidant products

An anti-oxidative and oxidative stress technology, applied in the field of medicine, can solve problems such as the application of Elabela polypeptides that have not been seen, and achieve the effect of reducing the generation of ROS and inhibiting oxidative stress damage.

Pending Publication Date: 2020-12-01
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is no application of Elabela polypeptide in anti-oxidation

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  • Application of Elabela polypeptide in preparation of antioxidant products
  • Application of Elabela polypeptide in preparation of antioxidant products
  • Application of Elabela polypeptide in preparation of antioxidant products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Elabela polypeptide inhibits the activity of NADPH enzyme activated by aldosterone

[0029] 1. Experimental method

[0030] Human renal tubular epithelial cells HK2 were planted in a 6-well plate, and cultured to 70% in a 37-degree incubator with DMEM-F12 medium (containing 10% serum and 1% double antibody).

[0031] The cell treatment is divided into 4 groups, which are respectively the control group (Ctrl): blank control, without any treatment;

[0032] Aldosterone treatment group (Aldo): 0.1 μM aldosterone treatment for 24 hours;

[0033] Elabela polypeptide treatment group (ELA): 1nM Elabela polypeptide treatment for 24 hours;

[0034] Aldosterone+Elabela polypeptide treatment group (Aldo+E peptide): After being treated with 1 nM Elabela polypeptide for one hour, 0.1 μM aldosterone was added to continue the treatment for 24 hours.

[0035] After 24 hours of treatment according to the group, the cells were collected. The relative amount of intracellular...

Embodiment 2

[0038] Example 2 Inhibition of Elabela Polypeptide on Aldosterone-Induced Active Oxygen Elevation

[0039] 1. Experimental method

[0040] Human renal tubular epithelial cells HK2 were planted in a 6-well plate, and cultured to 70% in a 37-degree incubator with DMEM-F12 medium (containing 10% serum and 1% double antibody).

[0041] Cell treatment was divided into 4 groups, namely control group (Ctrl), aldosterone treatment group (Aldo), Elabela polypeptide treatment group (ELA) and aldosterone+Elabela polypeptide treatment group (Aldo+E peptide). According to the grouping, after 1 nM of Elabela polypeptide was treated for one hour, 0.1 μM aldosterone was added to the corresponding group of cells, and after 24 hours of treatment, the cells were collected. The relative amount of intracellular reactive oxygen species was detected using a commercial reactive oxygen species detection kit.

[0042] 2. Experimental results

[0043] like figure 2 As shown, compared with the contr...

Embodiment 3

[0044] Example 3 Inhibition of the activity of NADPH enzyme activated by DOCA / salt by Elabela polypeptide

[0045] 1. Experimental method

[0046] Eighteen SD male rats were divided into three groups: control group (Ctrl), deoxycorticosterone combined with high salt group (DOCA / salt) and DOCA / salt+Elabela polypeptide high expression group in kidney (DOCA / salt-E peptide) .

[0047] Wherein, rats in the control group were given common feed and tap water;

[0048]Deoxycorticosterone synergistic high-salt group (DOCA / salt) rats were subcutaneously implanted with deoxycorticosterone capsules (150mg / rat), while adding 1% NaCl to drinking water for 3 weeks;

[0049] Deoxycorticosterone combined with high salt group + Elabela polypeptide high expression group in the kidney (DOCA / salt-E peptide), through intrarenal transfection of Elabela polypeptide high expression vector, and then subcutaneously implanted deoxycorticosterone capsules (150mg / rat), At the same time, 1% NACl was adde...

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Abstract

The invention provides an application of an Elabela polypeptide in preparation of antioxidant products. Research finds that the Elabela polypeptide can inhibit a pathway of activating NADPH oxidase bya risk factor aldosterone (Aldo) or an aldosterone analogue and reduce the activity of an active oxygen producing enzyme NADPH, so that the generation of active oxygen (ROS) is reduced, and oxidativestress injury induced by the active oxygen is inhibited. A new way is provided for researching and developing novel antioxidant products.

Description

technical field [0001] The invention belongs to the technical field of medicine. More specifically, it relates to the application of Elabela polypeptide in the preparation of antioxidant products. Background technique [0002] Skin aging is mainly caused by free radicals attacking healthy cells. If this happens for a long time, it will cause continuous damage to cells, loss of skin elasticity, dull complexion and other symptoms. Antioxidation is a process that directly acts on free radicals, or indirectly consumes substances that are prone to free radicals to prevent further reactions. Human skin is in continuous contact with the outside world, and ultraviolet rays, radiation, and air pollution are constantly infiltrating, generating free radicals, destroying skin collagen and elastic fibers, causing wrinkles, and causing pigmentation. Oxidative stress refers to a series of stress reactions caused by the imbalance of pro-oxidation and anti-oxidation homeostasis and excessi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P17/18A61K8/64A61Q19/08
CPCA61K38/1709A61P17/18A61K8/64A61Q19/08A61K2800/782
Inventor 王蕾
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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