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Application of 3, 4, 5-O-tricaffeoylquinic acid as cell protective agent in radiotherapy

A technology of caffeoylquinic acid and 5-O-, applied in the field of medicine, can solve the problems of limited clinical application and serious adverse reactions, and achieve the effects of inhibiting apoptosis and improving proliferation inhibition.

Pending Publication Date: 2020-12-15
福建众安药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the serious adverse reactions of these drugs, there are still limitations in clinical application.

Method used

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  • Application of 3, 4, 5-O-tricaffeoylquinic acid as cell protective agent in radiotherapy
  • Application of 3, 4, 5-O-tricaffeoylquinic acid as cell protective agent in radiotherapy
  • Application of 3, 4, 5-O-tricaffeoylquinic acid as cell protective agent in radiotherapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] In this example, normal colonic epithelial cells NCM460 were irradiated according to different radiation doses (0, 4, 6, 8 Gy), and the cell survival rates of NCM460 cells under different radiation doses were detected.

[0076] Method: In order to construct a radiation injury model, the normal colonic epithelial cells in this example were irradiated by Gammacell-40 low-dose rate research radiation instrument at room temperature at 0.70Gy / min, and the irradiation doses were 4Gy, 6Gy, and 8Gy, respectively. Recorded as irradiation groups 1, 2, and 3 in sequence; normal cultured colonic epithelial cells without irradiation were taken as the control group (NC group); 120 hours after irradiation, the cell viability of the irradiation group and the control group was detected to understand the cell viability of the cells. Survival rate and comparative observation. Each experiment was repeated three times. Cell viability was measured by MTT method, expressed as the mean ± SD o...

Embodiment 2

[0081] In this example, tCQA was added before the normal colonic epithelial cells NCM460 were irradiated, the cell viability was detected by the MTT method, and the cell proliferation was observed.

[0082] METHODS: Samples were dissolved in serum-free medium and added to final doses of tCQA containing 0 μg / mL, 2.5 μg / mL, 5 μg / mL, 10 μg / mL, 20 μg / mL and 40 μg / mL 4 hours before irradiation Then, they were irradiated with 4Gy, 6Gy and 8Gy respectively, and then the cells were cultured for 120h, and the cell proliferation was observed by MTT method. In this experiment, 0 μg / mL, normal cultured cells without radiation were used as the blank control group. Each experiment was repeated three times. The cell viability was detected by MTT method, and the results were as follows: figure 2 shown.

[0083] The data from these experiments were visually analyzed with Student's t-test to analyze the significance of the difference between the IR treatment group and the 0 μg / mL group: *p<...

Embodiment 3

[0088] In this example, the colony formation of normal colonic epithelial cells was used to determine the proliferation ability of the cells, so as to explore the effect of tCQA on IR-induced proliferation inhibition of normal colonic epithelial cells.

[0089] Method: 200 normal colonic epithelial cells were uniformly dispersed on a 24-well plate at a density of 200 per well, placed in media with final doses of tCQA of 0 μg / mL, 20 μg / mL and 40 μg / mL, and irradiated after 4 hours , the radiation dose was 8Gy, which were recorded as the experimental group (0μg / mL+8Gy), treatment group 1 (20μg / mL+8Gy) and treatment group 2 (40μg / mL+8Gy); then the cells were cultured for 7 days. In this experiment, 0 μg / mL, normal cultured cells without radiation were used as the blank control group. Each experiment was repeated three times. After the incubation, wash with PBS, visible colonies were fixed in 4% paraformaldehyde for 30 minutes, and stained with crystal violet for 20 minutes at ro...

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Abstract

The invention discloses an application of 3, 4, 5-O-tricaffeoylquinic acid as a cell protective agent in radiotherapy. The 3, 4, 5-O-tricaffeoylquinic acid has a protective effect on normal cells of adigestive system, an immune system and a hematopoietic system in the radiotherapy process, proliferation inhibition of common colonic epithelial cells caused by IR can be improved, and ionizing radiation induced cell cycle G2 / M retardation is reversed. According to the application disclosed by the invention, the 3, 4, 5-O-tricaffeoylquinic acid inhibits the production of reactive oxygen species (ROS) in cells, inhibits the mitochondrial pathway mediated by MAPKs generated by the ROS, and inhibits IR-induced apoptosis of normal colonic epithelial cells.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to the application of 3,4,5-O-tricaffeoylquinic acid as a cell protective agent in radiotherapy, which can affect the digestive system, immune system and hematopoietic system during radiotherapy. All have a protective effect. Background technique [0002] According to statistics, at present, 70% of cancer patients will receive radiotherapy during treatment, and radiotherapy is generally a local treatment method for treating tumors with ionizing radiation (IR). However, while radiotherapy kills cancer cells, it also causes nausea and loss of appetite in patients, and is accompanied by complications such as decreased white blood cells and decreased immune function, leading some patients to give up radiotherapy. [0003] In radiation therapy, high doses of radiation from uncontrolled release of radioactive material or intensive radiation therapy for cancer can also lead to gastrointe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/216A61P39/00A61P39/06
CPCA61K31/216A61P39/00A61P39/06
Inventor 刘润东邓元荣刘韧刘建文黄建明刘佳君
Owner 福建众安药业有限公司
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