A kind of large-scale cultivation method of Helicobacter pylori

A technology for Helicobacter pylori and large-scale cultivation, which is applied in the field of large-scale cultivation of Helicobacter pylori, can solve the problems of long cultivation period, small number of bacteria, and high requirements for cultivation conditions of Helicobacter pylori, so as to shorten the cultivation period and increase the number of bacteria. Effect

Active Publication Date: 2022-01-11
FOURTH HOSPITAL OF HEBEI MEDICAL UNIV +1
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Problems solved by technology

Due to the high requirements for Helicobacter pylori culture conditions, the number of bacteria obtained by traditional liquid culture and solid culture is small, the cost is high, and the culture period is long. It is difficult to achieve large-scale culture in vitro, which brings difficulties to the preparation of inactivated whole-bacteria vaccines.

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  • A kind of large-scale cultivation method of Helicobacter pylori

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Embodiment Construction

[0029] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts fall within the protection scope of the present invention.

[0030] Such as figure 1 Shown, a kind of Helicobacter pylori liquid-solid culture device, it comprises culture bottle, is arranged on the sealing cover of culture bottle top and is arranged on the heating platform 1 below culture bottle; Described culture bottle comprises solid culture part 2, liquid culture part 3 and an indicator ring 4 arranged between the solid culture part 2 and the liquid culture part 3. The solid culture part 2 is a truncated conica...

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Abstract

The present invention relates to a large-scale cultivation method of Helicobacter pylori, which utilizes a Helicobacter pylori liquid-solid culture device and includes the following steps: (A) coating a stirrer with 1mg / ml fibrinogen; (B) solid medium Preparation; (C) Helicobacter pylori was prepared into 1×10 9 Bacteria solution per ml, injected into Helicobacter pylori liquid-solid culture device, coated with bacteria, and cultured on solid medium; (D) Preparation of liquid medium; (E) Helicobacter pylori was first cultured on solid medium for 12 hours , inject the liquid medium, after the liquid medium is cultivated for 12 hours, continue to cultivate for 1-2 days under stirring, release the liquid medium, and collect Helicobacter pylori. The invention has short culture period, simple and convenient operation and large quantity of Helicobacter pylori, which provides basic conditions for preparing inactivated whole bacteria vaccine.

Description

technical field [0001] The invention belongs to a microorganism cultivation method, in particular to a large-scale cultivation method of Helicobacter pylori. Background technique [0002] Helicobacter pylori is a spiral-shaped, micro-anaerobic bacteria that requires very harsh growth conditions. It is the only microbial species known to survive in the human stomach. Due to the high requirements for the culture conditions of Helicobacter pylori, the number of bacteria obtained by traditional liquid culture and solid culture is small, the cost is high, and the culture period is long. It is difficult to achieve large-scale culture in vitro, which brings difficulties to the preparation of inactivated whole-bacteria vaccines. . Contents of the invention [0003] The object of the present invention is to provide a large-scale culture method of Helicobacter pylori with short culture period, simple and convenient operation and large number of bacteria. [0004] The present inven...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12M1/26C12M1/24C12M1/12C12M1/04C12M1/02C12M1/00C12R1/01
CPCC12N1/20C12M23/08C12M23/34C12M23/38C12M25/00C12M27/02C12M29/00C12M29/20C12M29/26C12M33/02C12M37/04C12M41/22
Inventor 单保恩赵连梅胡代伦
Owner FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
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