A kind of large-scale cultivation method of Helicobacter pylori

Abstract

The present invention relates to a large-scale cultivation method of Helicobacter pylori, which utilizes a Helicobacter pylori liquid-solid culture device and includes the following steps: (A) coating a stirrer with 1mg / ml fibrinogen; (B) solid medium Preparation; (C) Helicobacter pylori was prepared into 1×10 9 Bacteria solution per ml, injected into Helicobacter pylori liquid-solid culture device, coated with bacteria, and cultured on solid medium; (D) Preparation of liquid medium; (E) Helicobacter pylori was first cultured on solid medium for 12 hours , inject the liquid medium, after the liquid medium is cultivated for 12 hours, continue to cultivate for 1-2 days under stirring, release the liquid medium, and collect Helicobacter pylori. The invention has short culture period, simple and convenient operation and large quantity of Helicobacter pylori, which provides basic conditions for preparing inactivated whole bacteria vaccine.

Description

technical field [0001] The invention belongs to a microorganism cultivation method, in particular to a large-scale cultivation method of Helicobacter pylori. Background technique [0002] Helicobacter pylori is a spiral-shaped, micro-anaerobic bacteria that requires very harsh growth conditions. It is the only microbial species known to survive in the human stomach. Due to the high requirements for the culture conditions of Helicobacter pylori, the number of bacteria obtained by traditional liquid culture and solid culture is small, the cost is high, and the culture period is long. It is difficult to achieve large-scale culture in vitro, which brings difficulties to the preparation of inactivated whole-bacteria vaccines. . Contents of the invention [0003] The object of the present invention is to provide a large-scale culture method of Helicobacter pylori with short culture period, simple and convenient operation and large number of bacteria. [0004] The present inven...

AI Technical Summary

Problems solved by technology

Due to the high requirements for Helicobacter pylori culture conditions, the number of bacteria obtained by traditional liquid culture and solid culture is small, the cost is high, and the culture period is long. It is difficult to achieve large-scale culture in vitro, which brings difficulties to the preparation of inactivated whole-bacteria vaccines.

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