Micro-channel temperature control device and method for nucleic acid detection amplification reaction

Abstract

The invention discloses a micro-channel temperature control device and method for a nucleic acid detection amplification reaction, and relates to a micro-channel temperature control device and method.The device comprises a nucleic acid amplification reaction module, a heating/cooling module, a micro-channel cooling module and a microcontroller module, wherein the nucleic acid amplification reaction module and the micro-channel cooling module are respectively mounted on the upper surface and the lower surface of the heating/cooling module; the nucleic acid amplification reaction module comprises a reaction plate, reaction columns and reaction tubes; the heating/cooling module is attached to the bottom of the reaction plate, and heating/cooling and temperature control of the reaction moduleare achieved through the microcontroller module; the micro-channel cooling module comprises a micro-channel plate, a micro-channel cover plate, a liquid tube, a cooling fan, a condenser, a liquid storage device and a water pump; and thermocouples are arranged at outlets of the reaction columns, a thermoelectric refrigeration sheet and the condenser and used for collecting temperature signals. Anouter-layer thermal insulation material is not used, so that the temperature distribution uniformity is improved, the cost is reduced, and the production operation convenience is improved. The power of the thermoelectric refrigeration sheet is improved and the heating/cooling rate of the reaction module is improved.

Description

technical field [0001] The invention relates to a microchannel temperature control device and method, in particular to a microchannel temperature control device and method for nucleic acid detection amplification reaction. Background technique [0002] In recent years, with the development of gene technology, nucleic acid detection has played an increasingly important role. Clinically, through nucleic acid detection, lesions can be detected in advance and treated as soon as possible, thereby improving the cure rate. At present, infectious diseases are prevalent and various viruses are raging, seriously affecting people's health. The new crown pneumonia epidemic has caused millions of people to be infected. COVID-19 has a long incubation period and there are asymptomatic infections, which pose great challenges to the prevention, control and treatment of the epidemic. At present, the most important means of epidemic prevention is large-scale nucleic acid testing to achieve ...

AI Technical Summary

Problems solved by technology

At present, the nucleic acid detection equipment on the market adopts the heat dissipation method of thermoelectric cooling sheets and fins. Due to the low heat dissipation efficiency of the fins, only low-power thermoelectric cooling sheets can be used, resulting in the temperature rise / fall rate of the samples in the reaction module. less than required

Especially in the cooling process, the hot end of the thermoelectric cooling chip generates a lot of heat when it is working, and the fins cannot export the heat in time, causing the heat to flow back to the cold end, further reducing the cooling effect, and the cooling rate is far from meeting the current detection requirements

On the other hand, due to heat exchange between the reaction columns and reaction tubes around the reaction module and the surrounding air, the temperature of each sample is inconsistent, resulting in inconsistent nucleic acid concentrations after sample amplification, which seriously affects the detection sensitivity and accuracy.

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