Method for determining total lignans in Shuxuening injection
A technology of total lignans and determination methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve problems such as measurement result errors
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[0055] The assay method of total lignans in Shuxuening injection provided by the invention is investigated below in conjunction with test data:
[0056] 1. Specificity
[0057] Such as figure 1 It can be seen that both pinoresinol diglucoside and the test solution exhibit maximum absorption at UV277nm, and sorbitol and ginkgolides do not interfere with the determination of total lignans.
[0058] 2. Precision
[0059] 1. Repeatability
[0060] Get Shuxuening injection sample 2 to prepare the test solution according to the method of S2 in Example 1, parallel 6 parts, record the absorbance difference ΔA at UV277nm and 370nm, and calculate the test solution according to the standard curve of Example 1 The concentration (mg / L) of pinoresinol diglucoside in middle; The results are shown in Table 1.
[0061] Table 1
[0062]
[0063] 2. Instrument precision
[0064] Continuous measurement concentration is the pinoresinol diglucoside reference substance solution of 65.992mg / ...
Embodiment 1
[0090] This embodiment provides a method for assaying total lignans in Shuxuening injection, including:
[0091] S1, drawing a standard curve: accurately weigh pinoresinol diglucoside, add 10% methanol solution (volume ratio) to make a solution containing 0.4 mg per 1 ml, as a reference substance solution;
[0092] Accurately measure the reference substance solution 0.2mL, 0.4mL, 0.5mL, 0.8mL, 1.0mL, 2.0mL to a 5mL volumetric flask, add 10% methanol solution (volume ratio) to dilute to the mark; Measure the absorbance difference ΔA of different concentrations of reference substance solutions at 277nm and 370nm wavelengths; take the reference substance solution concentration (mg / L) as the abscissa (x), and record the absorbance difference ΔA at UV277nm and 370nm as the ordinate (y ), draw a standard curve;
[0093] S2. Prepare the test solution: accurately measure 2.0mL Shuxuening injection into a 5mL volumetric flask, add water to dilute to the mark, and shake well; load the ...
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