Method for determining total lignans in Shuxuening injection

A technology of total lignans and determination methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve problems such as measurement result errors

Pending Publication Date: 2020-12-11
HEILONGJIANG ZBD PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to accurately measure the content of total lignans in Shuxuening Injection, the present invention explores the method of improving the measurement accuracy, so as to solve the problems such as large errors in measurement results

Method used

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  • Method for determining total lignans in Shuxuening injection
  • Method for determining total lignans in Shuxuening injection
  • Method for determining total lignans in Shuxuening injection

Examples

Experimental program
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experiment example

[0055] The assay method of total lignans in Shuxuening injection provided by the invention is investigated below in conjunction with test data:

[0056] 1. Specificity

[0057] Such as figure 1 It can be seen that both pinoresinol diglucoside and the test solution exhibit maximum absorption at UV277nm, and sorbitol and ginkgolides do not interfere with the determination of total lignans.

[0058] 2. Precision

[0059] 1. Repeatability

[0060] Get Shuxuening injection sample 2 to prepare the test solution according to the method of S2 in Example 1, parallel 6 parts, record the absorbance difference ΔA at UV277nm and 370nm, and calculate the test solution according to the standard curve of Example 1 The concentration (mg / L) of pinoresinol diglucoside in middle; The results are shown in Table 1.

[0061] Table 1

[0062]

[0063] 2. Instrument precision

[0064] Continuous measurement concentration is the pinoresinol diglucoside reference substance solution of 65.992mg / ...

Embodiment 1

[0090] This embodiment provides a method for assaying total lignans in Shuxuening injection, including:

[0091] S1, drawing a standard curve: accurately weigh pinoresinol diglucoside, add 10% methanol solution (volume ratio) to make a solution containing 0.4 mg per 1 ml, as a reference substance solution;

[0092] Accurately measure the reference substance solution 0.2mL, 0.4mL, 0.5mL, 0.8mL, 1.0mL, 2.0mL to a 5mL volumetric flask, add 10% methanol solution (volume ratio) to dilute to the mark; Measure the absorbance difference ΔA of different concentrations of reference substance solutions at 277nm and 370nm wavelengths; take the reference substance solution concentration (mg / L) as the abscissa (x), and record the absorbance difference ΔA at UV277nm and 370nm as the ordinate (y ), draw a standard curve;

[0093] S2. Prepare the test solution: accurately measure 2.0mL Shuxuening injection into a 5mL volumetric flask, add water to dilute to the mark, and shake well; load the ...

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Abstract

The invention relates to the technical field of detection of active ingredients of ginkgo leaves, in particular to a method for determining total lignans in a Shuxuening injection. The determination method adopts ultraviolet dual-wavelength spectrophotometry, and comprises the following steps of: providing a Shuxuening injection; removing flavonoid components in the Shuxuening injection; and detecting the Shuxuening injection without the flavonoid components as a test solution. The method provided by the invention can accurately determine the content of the total lignans in the Shuxuening injection, so that the content of the total lignans in the Shuxuening injection is effectively controlled, and the uniformity and stability among batches of products are ensured.

Description

technical field [0001] The invention relates to the technical field of detection of ginkgo biloba active components, in particular to a method for determining total lignans in Shuxuening injection. Background technique [0002] Shuxuening injection is a traditional Chinese medicine preparation, which is a yellow clear liquid and is mainly used for cardiovascular diseases. The function is to dilate blood vessels and improve microcirculation. For ischemic cardiovascular and cerebrovascular diseases, coronary heart disease, angina pectoris, cerebral embolism, cerebral vasospasm, etc. Studies have confirmed that this product can promote cerebral blood circulation, dilate cerebral blood vessels, increase cerebral blood flow, improve and protect brain cells; increase the content of neurotransmitters, reduce nerve cell damage; improve energy metabolism and nutrition of hypoxic brain cells, improve Hypoxia resistance of brain cells; increase red blood cell SOD activity, inhibit ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/74
CPCG01N30/02G01N30/06G01N30/14G01N30/74
Inventor 方同华闫久江陈玉梅林珊康宁韩冰佟美鸿武志立付英楠夏迎迎
Owner HEILONGJIANG ZBD PHARMA
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