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A combined detection kit for IgG, IGM and IGA antibodies of 2019-ncov and its detection method

A 2019-ncov, detection method technology, applied in the field of biomedicine, can solve the problems of qualitative and quantitative judgment, affecting the accuracy of the detection results of 2019-ncov infection, etc.

Active Publication Date: 2022-03-01
深圳爱信生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the fact that the existing 2019-nCoV antibody detection kits or other products cannot simultaneously accurately determine the IgG, IgM and IgA antibody subtypes of 2019-nCoV at the same time, which will affect the accuracy of the detection results of 2019-nCoV infection Problem, the present invention provides a combination detection kit for IgG, IgM and IgA antibodies of 2019-nCoV and its detection method

Method used

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  • A combined detection kit for IgG, IGM and IGA antibodies of 2019-ncov and its detection method
  • A combined detection kit for IgG, IGM and IGA antibodies of 2019-ncov and its detection method

Examples

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Effect test

Embodiment 1

[0056] A combined detection kit for 2019-nCoV IgG, IgM and IgA antibodies, including 2019-nCoV marker protein coated plate, sample diluent, washing solution, anti-human IgA antibody enzyme labeling solution, anti-human IgG antibody enzyme labeling work solution, anti-human IgM antibody enzyme-labeled working solution, chromogenic substrate, stop solution and positive control; the positive control is the complex of anti-2019-nCoV marker protein monoclonal antibody IgG-human IgA / IgG / IgM antibody.

[0057] Among them, the preparation method of the 2019-nCoV protein-coated plate is:

[0058] a. Antigen protein coating: Dilute 2019-nCoVS1 protein and 2019-nCoV N protein with coating solution (0.01M sodium phosphate solution, pH 7.4) to 1 μg / mL, then mix according to 1:1 volume ratio and add Into a polystyrene 96-well plate with high binding capacity, add 0.1 mL per well, seal the sealing film, and place in a 37°C thermostat for 2 hours;

[0059] b. Plate washing: Shake off the exc...

Embodiment 2

[0072] Using the kit in Example 1 to detect 11 inactivated 2019-nCoV antibody positive sera and 31 inactivated 2019-nCoV antibody negative sera, the specific detection method includes the following steps:

[0073] S1. Primary antibody incubation: Mix 11 positive sera and 31 negative sera to be tested with the sample diluent at a volume of 1:100 to obtain 42 diluted samples. Take 0.1 mL of the above 42 diluted samples and add them to the In the wells of the 2019-nCoV marker protein-coated plate, add 3 duplicate wells for each sample, and set up 3 groups of negative controls and 3 groups of positive controls, seal the sealing film, and incubate in a 37°C incubator for 30 minutes;

[0074] S2. Wash the plate for the first time: Discard the liquid in the wells of the 2019-nCoV marker protein-coated plate incubated with the primary antibody, dilute the washing solution 10 times, and wash the plate 3 times;

[0075] S3. Enzyme label incubation: add 0.1mL anti-human IgA antibody enzy...

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Abstract

The invention relates to the technical field of biomedicine, and specifically discloses a combined detection kit for IgG, IgM and IgA antibodies of 2019-nCoV and a detection method thereof. The IgG, IgM and IgA antibody combined detection kit for 2019-nCoV includes 2019-nCoV marker protein coated plate, sample diluent, washing solution, anti-human IgA antibody enzyme-labeled working solution, anti-human IgG antibody enzyme-labeled work solution, anti-human IgM antibody enzyme labeling solution, chromogenic substrate, stop solution and positive control. The kit of the present invention can detect IgG, IgM and IgA antibodies of 2019‑nCoV at the same time, solves the problems of poor accuracy and high rate of false negative results in single antibody detection results, greatly improves detection efficiency, and has extremely high Value.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a combined detection kit for IgG, IgM and IgA antibodies of 2019-nCoV and a detection method thereof. Background technique [0002] Enzyme-Linked ImmunoSorbent Assay (Enzyme-Linked ImmunoSorbent Assay, ELISA) has now become a commonly used method in the field of analytical chemistry, and is an immunoassay technique developed on the basis of Immunoenzymatic Techniques. [0003] Coronaviruses are a family of viruses known to cause colds as well as diseases such as Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). The new coronavirus (2019-nCoV) is a new strain of coronavirus that has never been found in humans before. It belongs to the genus β-coronavirus, and its genetic characteristics are significantly different from the aforementioned MERS and SARS. Common signs after human infection with coronavirus include respiratory symptoms, fever, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/577G01N33/58G01N33/545G01N33/543
CPCG01N33/6854G01N33/56983G01N33/577G01N33/581G01N33/545G01N33/54393G01N2333/165G01N2469/20
Inventor 王晓倩周帅张大准赵国萍王晓东张付献
Owner 深圳爱信生物技术有限公司
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