Aromatic polyketide compounds talaromyoxaones A and B, preparation method and application thereof
A technology of compounds and medicinal salts, applied in biochemical equipment and methods, methods based on microorganisms, fungi, etc., can solve few problems
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Embodiment 1
[0023] (1) The fermentation medium is prepared by the following method: by mass fraction, add 1% glucose, 1% starch, 0.1% KH to tap water 2 PO4, 0.1% MgSO 4 ·7H 2 O, 0.1% peptone, 3% sea salt, and adjust the pH of the medium to 6.5 with NaOH or HCl aqueous solution. Configure 50L of fermentation medium, and then divide into 1L Erlenmeyer flasks, about 300 mL of medium per bottle. Prepared by autoclaving at 115°C for 25 minutes.
[0024] (2) Preparation of fermentation broth and mycelium: Inoculate the fungus T.purpureogenus SCSIO 41517 on a flat plate containing PDA medium. After the fungus grows spores, transfer the spores and mycelium from the flat plate to In the Erlenmeyer flask containing the fermentation medium, culture it on a shaker at 28°C for 2-3 days to make a seed liquid, and inoculate the seed liquid into the fermentation medium with a pipette gun (300mL of fermentation medium is contained in a 1L Erlenmeyer flask), The fermentation broth and mycelia were harv...
Embodiment 2
[0037] The protein tyrosine phosphatase activity test of embodiment 2 compound Talaromyoxaones A and B
[0038] The human protein tyrosine phosphatase CDC25B, SHP2, MEG2, SHP1, TCPTP, CD45 or PTP1B genes were cloned into Escherichia coli (Escherichia coli), then expressed and purified. Enzyme inhibitory activity was measured using p-nitrophenylphosphate (pNPP) as a substrate in 96-well plates containing 100 μL of reaction mixture per well. Human recombinant CDC25B, SHP2, MEG2, SHP1, TCPTP, CD45 or PTP1B (0.05 μg) were added to 50 μL of reaction buffer containing 50 mM HEPES, 100 mM NaCl, 1 mM EDTA and 1 mM dithiothreitol (DTT) (pH6.5), and then The compound samples to be tested (compounds 1-2) were added to each 96-well plate. Na 3 VO 4 As a positive control, DMSO as a negative control was used to evaluate this high-throughput screening system. After 15 min pre-incubation at room temperature, 50 μL of buffer containing 50 mM pNPP was added and incubation was continued at 3...
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