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Method for analyzing tobacco fingerprint spectrum through comprehensive two-dimensional liquid chromatography, comprehensive two-dimensional liquid chromatography device and system

A two-dimensional liquid chromatography and fingerprint technology, applied in measuring devices, analytical materials, material separation, etc., can solve problems such as insufficient resolution and limited peak capacity, and achieve high detection efficiency, strong separation ability, and simple pretreatment Effect

Pending Publication Date: 2020-12-25
SHANGHAI TOBACCO GRP CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only conventional one-dimensional liquid chromatography has limited peak capacity and insufficient resolution, which has become a bottleneck restricting the development of tobacco fingerprints.

Method used

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  • Method for analyzing tobacco fingerprint spectrum through comprehensive two-dimensional liquid chromatography, comprehensive two-dimensional liquid chromatography device and system
  • Method for analyzing tobacco fingerprint spectrum through comprehensive two-dimensional liquid chromatography, comprehensive two-dimensional liquid chromatography device and system
  • Method for analyzing tobacco fingerprint spectrum through comprehensive two-dimensional liquid chromatography, comprehensive two-dimensional liquid chromatography device and system

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] 1. Experimental part

[0077] 1.1 Instrument parameters

[0078] A comprehensive two-dimensional liquid chromatograph equipped with an autosampler, two binary pumps, a column thermostat, a dual two-position four-way valve, and a diode array detector (Agilent, USA); a linear smoking machine (Borgwaldt, Germany) ); electronic balance (accuracy: 0.0001g, Swiss Mettler Toledo company); SW12H ultrasonic instrument (Swiss Sono Swiss company); Milli-Q pure water meter (U.S. Millpore company).

[0079] The mobile phase A1 of one-dimensional chromatography is 10mM Na 2 HPO 4 The aqueous solution (pH=7.0), the mobile phase B1 is methanol, the chromatographic column is Zorbax phenyl-hexyl (2.1×150mm, 5μm), the injection volume is 2μL, the column temperature is 25℃, and the flow rate is 0.1mL / min. Mobile phase gradient program: 0-30min, 2%-40%B; 35-40min, 70%B; 41-60min, 2%B.

[0080] The mobile phase A2 of the two-dimensional chromatography is 0.1% TFA aqueous solution (pH=2),...

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Abstract

The invention provides a method for analyzing a tobacco fingerprint spectrum through comprehensive two-dimensional liquid chromatography, a comprehensive two-dimensional liquid chromatography device and a system. The method comprises the following steps that after sample pretreatment is carried out on tobacco, a tobacco fingerprint spectrum is analyzed by adopting comprehensive two-dimensional liquid chromatography, a phenyl-hexyl column is adopted as a first-dimensional liquid chromatography, and a C18 column is adopted as a second-dimensional liquid chromatography. The system comprises a pretreatment device and a comprehensive two-dimensional liquid chromatography device which communicating with each other, wherein the comprehensive two-dimensional liquid chromatography device comprisesa first-dimensional chromatography unit, a double two-position four-way valve and a second-dimensional chromatography unit, the first-dimensional chromatography unit comprises a first-dimensional pump, a first-dimensional chromatographic column and a first-dimensional quantitative loop, the second-dimensional chromatography unit comprises a second-dimensional pump, a second-dimensional chromatographic column, a second-dimensional quantitative loop and a detector. According to the method and system, on the basis of comprehensive two-dimensional liquid chromatography (LC * LC), an LC * LC analysis platform is built, a tobacco comprehensive two-dimensional fingerprint spectrum analysis method is built, and comparative analysis of different types of tobacco samples is achieved.

Description

technical field [0001] The invention relates to the technical field of tobacco fingerprint analysis, in particular to a comprehensive two-dimensional liquid chromatography analysis method for tobacco fingerprints and a comprehensive two-dimensional liquid chromatography device and system. Background technique [0002] In recent years, with the rapid development of instrument analysis technology, detection means and computer information processing technology, tobacco fingerprint technology has also been developed rapidly. Tobacco is an agricultural product with various types of tobacco leaves, and there may be certain differences in tobacco leaf samples from different origins, parts, and years. Tobacco fingerprints can effectively provide method templates and technical platforms for tobacco quality control. Current research shows that thin-layer chromatography (TLC), gas chromatography (GC), high-performance liquid chromatography (HPLC), high-performance capillary electrophor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/20G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/20G01N30/8686G01N2030/062G01N2030/201
Inventor 陈敏董惠忠沈世豪王天南戚大伟王亮
Owner SHANGHAI TOBACCO GRP CO LTD
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