A high-speed evanescent field frequency shift super-resolution microscopic imaging system and imaging method

Abstract

The invention discloses a high-speed evanescent field frequency-shifting super-resolution microscopic imaging system and imaging method. An on-chip light-emitting device is used to simultaneously generate evanescent fields of three wavelengths transmitted in the same direction, and a color camera is used to simultaneously illuminate the three wavelengths in the same direction. The frequency-shifted images generated by the wavelength evanescent field are collected, and the three wavelengths correspond to the frequency-shifted signals of red, green, and blue bands, and each wavelength illumination has different frequency-shifted amounts. In the process of image reconstruction, the multi-wavelength information captured at the same time is separated and interpolated separately, thereby obtaining sample information with different frequency shifts and different illumination directions. Finally, image reconstruction is carried out, and the high-frequency and low-frequency spectra of different directions of the sample are stitched together using a stitching algorithm in the spectral space, and finally a high-resolution image that breaks through the optical diffraction limit of a traditional microscope is restored. In the case of sacrificing a certain image sampling rate, the present invention can effectively reduce the number of images collected by the evanescent field frequency shift super-resolution method and improve the imaging speed.

Description

technical field [0001] The invention relates to the field of super-resolution microscopy, in particular to a high-speed evanescent field frequency shift super-resolution microscopic imaging system and imaging method. Background technique [0002] The traditional optical system is limited by Abbe's diffraction limit, and its imaging resolution has a certain limit. In order to break through the resolution limit of the optical microscopy system, a series of fluorescent labeling and non-fluorescent labeling methods have been proposed. Among them, the frequency-shifting super-resolution microscopy imaging technology breaks the bandwidth limitation of traditional imaging systems and detector devices, and shows the advantages of fast and large field of view imaging. Frequency-shifting label-free super-resolution microscopy does not require fluorescent labels, and can achieve super-resolution imaging of non-biological samples, which has broad application prospects. [0003] The ev...

AI Technical Summary

Problems solved by technology

However, each image acquisition process requires a certain camera integration time, which limits the imaging speed of this imaging method and is not conducive to obtaining dynamic images with high frame rates.

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