Liquid chromatography joint detection method for vitamins A, D3 and E

Abstract

The invention relates to the field of detection, and particularly discloses a liquid chromatography joint detection method for vitamins A, D3 and E. The method comprises the following steps: (1) respectively preparing a vitamin A standard stock solution, a vitamin D3 standard stock solution and a vitamin E standard stock solution; (2) respectively sucking a proper amount of the vitamin A standardstock solution, the vitamin D3 standard stock solution and the vitamin E standard stock solution into a brown volumetric flask, and fixing the volume with methanol to obtain a standard working solution; (3) adding a to-be-tested sample into the brown volumetric flask, adding methanol, heating, carrying out ultrasonic extraction, cooling, and fixing the volume to obtain a to-be-tested sample solution; and (4) respectively carrying out liquid chromatography variable wavelength detection on the standard working solution and the to-be-tested sample solution. According to the joint detection method, the vitamins A, D3 and E in the to-be-detected sample can be extracted at the same time, the contents of the vitamins A, D3 and E are detected at the same time by controlling a mobile phase and a variable wavelength detection mode, the detection efficiency is improved, and the accuracy is high.

Description

technical field [0001] The application relates to the field of component detection, more specifically, it relates to a liquid chromatography joint detection method for vitamins A, D3 and E. Background technique [0002] Vitamins are a type of trace organic substances that humans and animals obtain from food to maintain normal physiological functions, and play an important role in the growth, metabolism, and development of the human body. For example, vitamin A can maintain normal vision, resist dry eye disease, maintain the integrity of epithelial structure cells, promote growth and development, and maintain normal immune function; for example, vitamin D can promote the absorption of calcium by the small intestinal mucosa, promote bone tissue calcification, and promote Reabsorption of calcium and phosphorus by renal tubules; vitamin E, for example, has strong antioxidant capacity, can delay aging, and regulate platelet adhesion and aggregation. Therefore, vitamin ingredient...

AI Technical Summary

Problems solved by technology

[0004] In view of the low detection efficiency of different vitamins in the prior art, the first purpose of this application is to provide a liquid chromatography joint detection method for vitamins A, D3, and E, which can simultaneously extract the vitamins in the sample to be detected. Vitamin A, D3, E, through the control of mobile phase and variable wavelength detection method, the content of vitamin A, D3, E can be detected at the same time, which improves the detection efficiency and high accuracy

Images