Method for detecting anthocyanin in mulberries based on Raman spectrum technology

Abstract

The invention discloses a method for detecting anthocyanin in mulberries based on a Raman spectrum technology. The method comprises the following steps: respectively collecting Raman spectra of cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside, geranium-3-O-glucoside, a mixed anthocyanin standard solution and a mulberry sample by adopting a Raman spectrometer; analyzing to obtain spectral information of anthocyanin, and determining Raman characteristic peaks; and allowing the collected mulberry sample Raman spectrum to be subjected to pretreatment and wavelength variable screening, and combininga mathematical model to establish an anthocyanin content prediction model. According to the method provided by the invention, the cyanidin- -3-O-glucoside and the geranium-3-O-glucoside can be quickly identified, and the anthocyanin in a mulberry sample can be qualitatively and quantitatively detected; the pretreatment method is good in applicability, the established mulberry anthocyanin quantitative model is high in prediction precision, and the method has the advantages of being high in detection speed, easy to operate, accurate in result and the like.

Description

technical field [0001] The invention relates to the fields of spectroscopy and rapid detection of fruit quality, in particular to a method for detecting anthocyanins in mulberries based on Raman spectroscopy. Background technique [0002] Anthocyanins are a kind of natural water-soluble flavonoid pigments, the most common ones are cyanidin, geranium, delphinidin, paeoniflorin, petuniasin and mallowin and other 6 kinds of anthocyanins as aglycone Anthocyanins formed by combining sugars and organic acids have the functions of protecting the human cardiovascular system, lowering blood sugar, protecting the liver, anti-cancer and improving eyesight. Mulberry has become a good raw material for processed food, health products and medicines because of its rich anthocyanins. Anthocyanins are unstable and susceptible to pH changes, light, heat, and certain metal ions, resulting in lightening of the color and reduction of physiological functions, making it difficult to maintain the q...

AI Technical Summary

Problems solved by technology

[0005] The main purpose of the present invention is to solve the problems that the traditional detection method is complex in operation, slow in detection speed, difficult to realize anthocyanin structure analysis and rapid identification, and the prediction accuracy of existing rapid detection technology needs to be improved. Method for technical detection of anthocyanins in mulberry

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