Method for establishing Caihong poplar regeneration system by taking leaves as explants

A technology of explants and poplar, applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve the problems of high differentiation rate, low pollution rate, difficulty in establishing the first-generation sterile line, etc., and achieve high-efficiency production Effect

Active Publication Date: 2021-02-19
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is very difficult to establish the first-generation sterile line, and the method of regeneration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for establishing a poplar regeneration system with leaves as explants, comprising:

[0033] 1. Selection and disinfection of explants: at the end of June, the selected surface area was 5cm 2 Left and right young leaves, shake the detergent for 10 minutes, and rinse with running water for 30 minutes. Shake with 75% alcohol for 1 minute, then shake with 0.1% mercuric chloride for 12 minutes, wash with sterile water 6 times to remove residual disinfectant. A total of 100 explants were processed;

[0034] 2. Induction of adventitious buds: cut off the edge of the detoxified leaf explants, and retain an area of ​​about 3cm 2 , the explants were inoculated in the induction medium MS+6-BA 1mg / L +NAA0.2 mg / L for adventitious bud induction, the culture temperature was controlled at 26°C, and cultured for 25 days, the culture conditions were: light time 14h / day, the light intensity was 1600lx; after 25 days, the explant contamination rate was 50%, and the induction r...

Embodiment 2

[0039] A method for establishing a poplar regeneration system with leaves as explants, comprising:

[0040] 1. Selection and disinfection of explants: at the end of June, the selected surface area was 5cm 2 Left and right young leaves, shake the detergent for 10 minutes, and rinse with running water for 30 minutes. Shake with 75% alcohol for 1 minute, then shake with 0.1% mercuric chloride for 12 minutes, wash with sterile water 6 times to remove residual disinfectant. A total of 100 explants were processed;

[0041] 2. Induction of adventitious buds: cut off the edge of the detoxified leaf explants, and retain an area of ​​about 3cm 2 , the explants were inoculated in the induction medium MS+6-BA 1mg / L +NAA0.2 mg / L for adventitious bud induction, the culture temperature was controlled at 26°C, and the red light was irradiated for 1 day, and the red light had a wavelength of 720nm The electromagnetic radiation, the light intensity is 4800lx; Then continue to cultivate for 2...

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Abstract

The invention belongs to the field of tissue culture propagation, and discloses a method for establishing a Caihong poplar regeneration system by taking leaves as explants. The method comprises the following steps that firstly, the explants are selected, then are disinfected with 0.1% of HgCl2 for 12 minutes and are inoculated into an induction culture medium for adventitious bud induction, the culture temperature is controlled within 24-26 DEG C, then culture is carried out for 25 days under the conditions that the illumination time is 10-14 h/d and the illumination intensity is 1400-1800 lx,then seedlings of the adventitious buds of the Caihong poplar are cut and transferred into a subculture medium for culture, and finally aseptic seedlings of the Caihong poplar are inoculated into a rooting culture medium for rooting culture for 25 days. According to the method, the problem of in-vitro rapid propagation of the Caihong poplar can be effectively solved.

Description

technical field [0001] The invention relates to a method for tissue culture and propagation of poplar, in particular to a method for establishing a regeneration system of poplar by using leaves as explants, and belongs to the technical field of seedling propagation of woody ornamental plants. Background technique [0002] Caihong Yang, belonging to the genus Populus of Salicaceae, is a new gene-induced budding variety of Populus americana 2025. The color of the branches and leaves in the growth period of the whole year is bright red-light yellow-orange-yellow green from top to bottom. At present, this variety mainly adopts two methods of cutting and grafting for plant propagation. The precautions required for cutting seedlings, preparation of cuttings, cutting time, cutting density and seedling management techniques in the process of growing seedlings by cuttings are basically the same as those of other poplar varieties. When grafting seedlings, the choice of rootstock is ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 束晓春庄维兵王忠张凤姣王涛王宁
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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