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Method for purifying an enveloped virus

An enveloped virus, carrier technology, applied in chemical instruments and methods, viruses, biochemical equipment and methods, etc., can solve problems such as not meeting the purity level

Pending Publication Date: 2021-02-19
PROBIOGEN AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The described methods often do not meet the required levels of purity for pharmaceutical products manufactured with continuous cells

Method used

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  • Method for purifying an enveloped virus
  • Method for purifying an enveloped virus
  • Method for purifying an enveloped virus

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Embodiment approach

[0075] As mentioned above, the inventors of the present patent application have unexpectedly established for the first time hydrophobic ion exchange mixed-mode chromatography for the purification of enveloped viruses. This is a fully scalable and repeatable process. The method allows purification of enveloped viruses in high yield and purity. In addition, the inventors of the present patent application found that the presence of arginine in the elution buffer further allows to increase the yield and purity of the virus. The high yield recovery of enveloped viruses was very surprising since arginine is used in the art to inactivate enveloped viruses (EP2350271B1). The virus purification method of the present invention uses mixed-mode chromatography in bind-elute-mode, which is suitable for overcoming practical shortcomings (e.g., in purity, speed, recovery, versatility) in the purification of enveloped viruses. , robustness and / or scalability shortcomings). The method is sui...

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Abstract

The present invention relates to a method for purifying an enveloped virus. The present invention further relates to an enveloped virus or a plurality of enveloped viruses obtainable by said method.

Description

technical field [0001] The present invention relates to a method for purifying enveloped viruses. The invention further relates to an enveloped virus or enveloped viruses obtainable by said method. Background technique [0002] Today, in addition to monoclonal antibodies, new biopharmaceutical products based on viral particles hold great promise. These viral particles could be used in gene therapy or immunization. Broad-spectrum applications, especially in the treatment or prevention of cancer or infectious diseases, combined with expanding medical markets, are driving improvements in the production processes of gene therapy vectors and viral vaccines. [0003] The use of well-characterized animal cell lines and scalable production systems has allowed for greatly improved titers and product quality of native and recombinant viruses. However, much less has been invested in downstream purification processes of viral particles. [0004] Process-related contaminants (e.g., b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/02
CPCB01D15/327B01D15/361B01D15/3847C12N7/00C12N2710/24151C12N2760/18151C12N15/113
Inventor 福尔克·桑迪希迈克尔·米勒斯文·克鲁格纳
Owner PROBIOGEN AG