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Application of statin compounds in preparation of drugs for promoting regeneration function of mammal spermatogonial stem cells

A technology of spermatogonial stem cells and mammals, applied in the direction of drug combinations, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve problems such as inability to obtain offspring, and achieve the effect of avoiding long cycles and mature applications

Pending Publication Date: 2021-03-12
首玺(广州)医疗科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the inability to obtain healthy and mature male gametes, traditional assisted reproductive technology cannot be implemented for treatment. At present, the only clinical solution to this problem is to use donor sperm from sperm banks to reproduce, so they cannot obtain real biological offspring

Method used

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  • Application of statin compounds in preparation of drugs for promoting regeneration function of mammal spermatogonial stem cells
  • Application of statin compounds in preparation of drugs for promoting regeneration function of mammal spermatogonial stem cells
  • Application of statin compounds in preparation of drugs for promoting regeneration function of mammal spermatogonial stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 The promotion effect of different concentrations of lovastatin on the regeneration function of mouse spermatogonial stem cells through in vitro culture

[0060] (1) Preparation of medium for culturing spermatogonial stem cells in vitro (take 50mL as an example)

[0061]

[0062]

[0063] (2) Prepare culture dishes and feeder cells for inoculation of spermatogonial stem cells

[0064] Fetal mouse fibroblasts treated with mitomycin C were inoculated in each culture well of a 96-well culture plate as feeder cells for culturing mouse spermatogonial stem cells.

[0065] (3) Effects of different concentrations of lovastatin on spermatogonial stem cells cultured in vitro

[0066] The mouse spermatogonial stem cells were inoculated in the culture wells prepared with feeder cells in advance, and the inoculation density was 10 4 Cells / well, add different concentrations of lovastatin from 0 μM to 20 μM in the culture medium; cells are placed in 5% CO 2 , In a co...

Embodiment 2

[0070] Example 2 The promotion effect of various statin compounds on the regeneration function of mouse spermatogonial stem cells through in vitro culture

[0071] (1) Preparation of medium for culturing spermatogonial stem cells in vitro (take 50mL as an example)

[0072]

[0073]

[0074] (2) Prepare culture dishes and feeder cells for inoculation of spermatogonial stem cells

[0075] Fetal mouse fibroblasts treated with mitomycin C were inoculated in each culture well of a 96-well culture plate as feeder cells for culturing mouse spermatogonial stem cells.

[0076] (3) Effects of various statin compounds on spermatogonial stem cells cultured in vitro

[0077] The mouse spermatogonial stem cells were inoculated in the culture wells prepared with feeder cells in advance, and the inoculation density was 10 4 Cells / well, add different kinds of statin compounds in sequence in the culture medium: lovastatin, pravastatin sodium, fluvastatin sodium, atorvastatin calcium, pi...

Embodiment 3

[0081] Example 3 Statins can maintain the long-term culture of mouse spermatogonial stem cells under the condition of no exogenous addition of cytokine GDNF in vitro

[0082] (1) Preparation of medium for culturing spermatogonial stem cells in vitro (take 50mL as an example)

[0083]

[0084]

[0085] (2) Prepare culture dishes and feeder cells for inoculation of spermatogonial stem cells

[0086] Fetal mouse fibroblasts treated with mitomycin C were inoculated in each well of a 12-well culture plate as feeder cells for culturing mouse spermatogonial stem cells.

[0087] (3) The effects of statins on the cultivation and passage of spermatogonial stem cells in vitro

[0088] The mouse spermatogonial stem cells were inoculated in the culture wells prepared with feeder cells in advance, and the inoculation density was 2×10 5 Cells / well, add 5 μM lovastatin to the culture medium; set up two control groups at the same time, that is, add an equal volume of lovastatin solvent...

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Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to application of statin compounds in preparation of drugs for promoting the regeneration function of mammal spermatogonial stem cells. The statin compounds can be applied to preparation of drugs for promoting the regeneration function of mammal spermatogonial stem cells, a novel drug choice is providedfor prevention and / or treatment of male sterility, statin compounds are mature and safe in application due to new use of old drugs, can be rapidly converted into clinic, and the period of new drug research and development is avoided; specifically, the statin compounds can promote regeneration of the mammal spermatogonial stem cells in an in-vitro culture mode or a mode of being directly applied tothe body, and can also promote continuous generation of sperms by firstly performing in-vitro culture to promote regeneration of the mammal spermatogonial stem cells and then transplanting the spermatogonial stem cells into the body. And a plurality of options are provided for prevention and / or treatment modes of male sterility.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of statin compounds in the preparation of drugs for promoting the regeneration function of mammalian spermatogonial stem cells. Background technique [0002] At present, infertility in the world accounts for about 15% of couples of childbearing age, and it is increasing year by year. Among them, male factor-induced infertility accounts for about 50%, and the reason is mostly caused by gametogenesis disorders. Due to the inability to obtain healthy and mature male gametes, traditional assisted reproductive technology cannot be implemented for treatment. Currently, the only clinical solution to this problem is to use donor sperm from sperm banks for reproduction, so they cannot obtain biologically meaningful offspring. [0003] Spermatogonial stem cells are a kind of undifferentiated germ cells originating from primordial germ cells, which are distrib...

Claims

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Application Information

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IPC IPC(8): A61K31/366A61P15/00A61P15/08
CPCA61K31/366A61P15/00A61P15/08
Inventor 赵小阳李朝晖姚昭锴罗芳万聪常港
Owner 首玺(广州)医疗科技有限责任公司
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