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Mirror protein information storage and encoding technology

A technology for information storage and mirroring, applied in the field of biological information, can solve the problems of difficult chemical preparation of DNA, inability to save information, copy wrong information, etc., to achieve the effect of simple and convenient information writing and increase the complexity of coding

Active Publication Date: 2021-11-12
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

DNA storage and encoding technology often encounters difficulties in DNA chemical preparation, but the use of DNA template amplification is prone to replication errors and information loss
More importantly, biomolecules such as DNA and proteins based on natural nucleotides or amino acids have poor biological stability, are susceptible to microbial contamination and degrade and disappear over time, and cannot achieve information under specific environmental conditions such as long-term and natural conditions. save
In addition, biological information molecules such as DNA and protein are also easily contaminated by DNA and protein from natural sources.

Method used

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  • Mirror protein information storage and encoding technology
  • Mirror protein information storage and encoding technology
  • Mirror protein information storage and encoding technology

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Mirror protein fragment preparation and sequencing

[0040] 1.1. Preparation of mirror image protein fragments (coding information writing)

[0041] Using the Fmoc solid-phase peptide synthesis method, prepare the following mirror image peptide fragments, refer to figure 1 (a):

[0042] A1 mirror image peptide fragment:

[0043] h 2 N-fGhvwekapGshhwGk(Biotin)-CONH 2 (wherein, k(Biotin) means biotinylated lysine). The specific operation steps are as follows:

[0044] First, add Rink Amide-AM resin to the peptide synthesis tube, use dimethylformamide (DMF) / dichloromethane (DCM) (volume ratio 1:1) to swell, and add benzotriazole-N, N,N',N'-Tetramethyluronium hexafluorophosphate (HBTU, 3.8 equivalents) / Hydroxybenzotriazole (HOBt, 4 equivalents) / Diisopropylethylamine (DIEA, 8 equivalents ) / Fmoc-protected target peptide C-terminal first amino acid (4 times equivalent) was dissolved in DMF, added to the peptide synthesis tube containing resin to react for 2 hours, the r...

Embodiment 2

[0061] Mirror protein encoding library for storing and encoding Chinese characters, see the operation process Figure 7 and 8 .

[0062] 2.1, "Chinese" hexadecimal encoding rules based on mirror protein (information encoding)

[0063] Using the mirror protein coding system, based on the hexadecimal national standard code, it is used for information storage and coding of Chinese words. As shown in Table 1, taking "Tsinghua University", "China University of Science and Technology", "Shanghai University" and "mirror protein" as examples, the present invention defines a mirror protein coding rule, as shown in Table 1, All characters in hexadecimal corresponding to unique d-amino acid coding sequences are listed. This artificially designed encoding rule can further increase the privacy and security of the encoded information. According to the rules in Table 1, the mirror protein coding sequences corresponding to the obtained Chinese information such as "Tsinghua University", "C...

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Abstract

The invention discloses a method for storing and encoding information by using a mirror image protein, comprising the following steps: Step 1: Coding a computer file; Step 2: Converting the coded sequence into D-type amino acid and glycine Mirror protein sequence; step 3: segment the mirror protein sequence, and add numbering information sequence, verification information sequence and information sequence that can realize random data access at both ends of each mirror protein fragment sequence obtained; step 4: Mirror protein fragments are made into mirror protein information storage materials. Different from the traditional silicon-based storage method, the method of the present invention uses the encoded mirror protein containing specific information, which has the advantages of high information storage density, long storage time, easy access to storage media, unlimited shape of storage and encoding media, and tolerance to storage conditions High and maintenance-free, it can be used for high information density, long storage time, and strong plasticity information storage and coding, and will have broad application prospects in massive information storage and everything coding.

Description

technical field [0001] The invention relates to the field of biological information. Specifically, the present invention relates to Mirror protein information storage and encoding technology. Background technique [0002] The storage and encoding of information enables the inheritance and dissemination of human social civilization. The ancient Chinese ancestors used the media preserved to this day to record their works and art, making the splendid Chinese civilization spread through the ages. For example, oracle bone inscriptions stored and encoded on the surface of turtle shells document the late Shang civilization from 1200 BC to 1050 BC. Up to now, the information age marked by computers (including personal smart terminals, Internet of Things and genetic databases, etc.) has produced huge amounts of information, and the amount of information storage has shown an exponential growth trend. The development of human civilization has entered the digital world. [0003] The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B15/20G16B30/10G16B50/00C07K1/04C07K1/06G01N33/68
CPCC07K1/04C07K1/06G01N33/6818G16B15/20G16B30/10G16B50/00
Inventor 刘磊郑基深胡宏岗田长麟梁军石威巍李影
Owner TSINGHUA UNIV
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